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ProteomicsImplementing 1.5 mm internal diameter columns into microflow LC-MS bottom-up proteomics
In this study, we systematically evaluated the 1.5 mm i.d. column and compared its performance with 1.0 mm i.d. columns in analyzing protein samples of varying complexity using a conventional chromatographic system.
ORGANISM(S): Francisella tularensis subsp. holarctica (strain LVS) Homo sapiens (Human) 
2025-02-11 | PXD057525 | Pride
Proteomics Chromatography Columns Lc/ms Inner diameter 
ProteomicsDeep single-shot nanoLCMS proteome profiling with 1500 bar UHPLC system, long fully porous columns, and HRAM MS
This study demonstrates how the latest UHPLC (ultra-high-performance liquid chromatography) technology can be combined with high-resolution accurate-mass (HRAM) mass spectrometry (MS) and long columns packed with fully porous particles to improve bottom-up proteomics analysis with nano-flow liquid c...
ORGANISM(S): Homo sapiens (Human) 
2022-09-12 | PXD035665 | Pride
Human Lcms Cells Orbitrap 
ProteomicsSelf-Packed Capillary 300 µm Columns for Deep and Sensitive Proteome Analysis.
Nanoflow chromatography remains the standard workflow for proteomics. Capillary flow chromatography applications are mainly limited to fast acquisitions, likely due to the absence of commercially available long (> 15 cm), 300 µm inner diameter columns. We present a methodology for packing 300 µm dia...
ORGANISM(S): Homo sapiens (Human) 
2026-02-07 | PXD067260 | Pride
Capillary flow Caplc Lc 
ProteomicsInvestigating the mechanism of action of IVIG in DSS-induced colitis mice through proteomics.
In this study, a mouse model of dextran sulfate sodium (DSS)-induced colitis was employed to investigate the role and mechanism of IVIG by proteomics.
ORGANISM(S): Mus musculus (Mouse) 
2026-07-28 | PXD068491 | Pride
Ivig dss-induced colitis mice proteomics. 
ProteomicsiEvaluation of spin columns for human plasma depletion to facilitate MS-based proteomics analysis of SARS-cov-2 positive plasman columns for human plasma depletion to facilitate MS-based proteomics analysis of SARS-cov-2 positive plasma
the benefit on the number of identified proteins is well documented and the depletion step remains widely used in in-depth proteomics. Recently, single-use depletion spin columns have been developed (i.e High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, Thermo) which greatly reduces ...
ORGANISM(S): Homo sapiens (Human) Macaca mulatta (Rhesus macaque) 
2021-07-30 | PXD023148 | Pride
Proteomics Mass spectrometry Plasma Depletion Sars-cov-2 Human Spin-column 
ProteomicsHighly Multiplex Targeted Proteomics Enabled by Real-Time Chromatographic Alignment
Targeted mass spectrometry methods produce high quality quantitative data in terms of limits of detection and dynamic range, at the cost of a substantial compromise in throughput compared to methods such as data independent and data de-pendent acquisition. The logistical and experimental issues inhe...
ORGANISM(S): Homo Sapiens 
2021-01-24 | PXD018675 | panorama
UnknownMachine learning-based prediction of the axial load capacity of UHPC strengthened reinforced concrete columns: A comparative analysis.
Not available
S-EPMC12779125 | biostudies-literature
TranscriptomicsLong noncoding RNA expression during human B-cell development
Long noncoding RNAs (lncRNAs) have emerged as important regulators of diverse cellular processes, but their roles in the developing immune system are poorly understood. In this study, we analysed lncRNA expression during human B-cell development by array-based expression profiling of eleven distinct...
ORGANISM(S): Homo sapiens 
2016-02-09 | E-GEOD-69033 | biostudies-arrayexpress
UnknownImplementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh pressure liquid chromatography columns.
Not available
S-EPMC5501974 | biostudies-literature
TranscriptomicsA Long Non-coding RNA, LncMyoD, Regulates Skeletal Muscle Differentiation by Blocking IMP2-mediated mRNA Translation
Increasing evidence suggests that Long non-coding RNAs (LncRNAs) represent a new class of regulators of stem cells. However, the roles of LncRNAs in stem cell maintenance and myogenesis remain largely unexamined. For this study, hundreds of novel intergenic LncRNAs were identified that are expresse...
ORGANISM(S): Mus musculus 
2015-08-11 | E-GEOD-68842 | biostudies-arrayexpress
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