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Soil columns Raw sequence reads
Reversed-phase high performance liquid chromatography is the most commonly applied separation technique in mass spectrometry-based proteomics. Particle-packed capillary columns are predominantly used for nano-flow systems. Despite being the broadly applied standard for many years capillary columns a...
ORGANISM(S): Homo sapiens (Human) 
2021-04-28 | PXD024296 | Pride
In this study, we systematically evaluated the 1.5 mm i.d. column and compared its performance with 1.0 mm i.d. columns in analyzing protein samples of varying complexity using a conventional chromatographic system.
ORGANISM(S): Francisella tularensis subsp. holarctica (strain LVS) Homo sapiens (Human) 
2025-02-11 | PXD057525 | Pride
Mixed microbes in columns (Thiobacillus sp.) Genome sequencing and assembly
High-resolution capillary chromatography is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We report a simple and low-cost FlashPack protocol for preparation of ultra-high performance capillary columns of > 50 cm length in less than 1 h using low-pressure equipment...
ORGANISM(S): Homo sapiens (Human) 
2019-01-21 | PXD009519 | Pride
Sulfate fertilization of ryegrass soil columns negatively affects microbial communities and nematodes but improves plant sulfur supply and growth
Nanoflow chromatography remains the standard workflow for proteomics. Capillary flow chromatography applications are mainly limited to fast acquisitions, likely due to the absence of commercially available long (> 15 cm), 300 µm inner diameter columns. We present a methodology for packing 300 µm dia...
ORGANISM(S): Homo sapiens (Human) 
2026-02-07 | PXD067260 | Pride
Surface water, storm water and storm water inoculated columns and controls
the benefit on the number of identified proteins is well documented and the depletion step remains widely used in in-depth proteomics. Recently, single-use depletion spin columns have been developed (i.e High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, Thermo) which greatly reduces ...
ORGANISM(S): Homo sapiens (Human) Macaca mulatta (Rhesus macaque) 
2021-07-30 | PXD023148 | Pride
archaeal communities along the water columns of the South China Sea Metagenome
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