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ProteomicsSelf-Packed Capillary 300 µm Columns for Deep and Sensitive Proteome Analysis.
Nanoflow chromatography remains the standard workflow for proteomics. Capillary flow chromatography applications are mainly limited to fast acquisitions, likely due to the absence of commercially available long (> 15 cm), 300 µm inner diameter columns. We present a methodology for packing 300 µm dia...
ORGANISM(S): Homo sapiens (Human) 
2026-02-07 | PXD067260 | Pride
Capillary flow Caplc Lc 
UnknownIsolation of cDNA encoding the catalytic site of phosphatidylinositol-specific phospholipase C from Coffea arabica L.: Recombinant expression and peptide purification.
Not available
S-EPMC2633734 | biostudies-literature
ProteomicsHigh-performance proteomics using nano-, capillary- and micro-flow chromatographic separations
In this study, we systematically evaluated the performance of μLC (50, 10 μL/min), capLC (5, and 1.5 μL/min), and nLC (0.3 μL/min ) flow-rates for proteome analysis using a Vanquish Neo HPLC coupled with a Q Exactive HF-X mass spectrometer. Serial dilutions of HeLa cell line digests were used for b...
ORGANISM(S): Homo sapiens (Human) 
2025-09-03 | PXD062536 | Pride
Kinobeads Lc-ms Phospho-proteomics Capillary-flow chromatography 
UnknownRelative quantitation of neuropeptides over a thousand-fold concentration range.
Not available
S-EPMC3515743 | biostudies-literature
TranscriptomicsFunctional reconstitution of adrenocortical transdifferentiation from human pluripotent stem cells
Proper prenatal adrenal cortex development is essential to prevent life-threatening adrenal insufficiency. This process relies on self-renewing progenitor cells within a capsule (Cap)-derived niche of the definitive zone (DZ) and trans-differentiation into cortisol-producing transitional and androge...
ORGANISM(S): Homo sapiens 
2026-05-05 | GSE291218 | GEO
UnknownSodium laurate, a novel protease- and mass spectrometry-compatible detergent for mass spectrometry-based membrane proteomics.
Not available
S-EPMC3610932 | biostudies-literature
UnknownHigh-Performance Proteomics Using Nano-, Capillary-, and Microflow Chromatographic Separations.
Not available
S-EPMC12501992 | biostudies-literature
ProteomicsPME1 interactome - Relevance Rank Platform (RRP) for Functional Filtering of High Content Protein-Protein Interaction Data
For a single step AP-MS identification of PME1 interactome was established a cell line stably expressing either Strep-tag alone or Strep_PME-1 fusion protein. After Strep-tag affinity purification, proteins were separated on SDS-PAGE gel, silver stained and protein bands that showed visually...
ORGANISM(S): Homo sapiens (Human) 
2015-12-08 | PXD002637 | Pride
Human 
UnknownEnrichment and proteomic analysis of plasma membrane from rat dorsal root ganglions.
Not available
S-EPMC2780401 | biostudies-literature
UnknownHand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements.
Not available
S-EPMC9774231 | biostudies-literature
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