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ProteomicsMetabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment
Epilepsy is characterized by hypersynchronous neuronal discharges, which are associated with an increased cerebral metabolic rate of oxygen and ATP demand. Uncontrolled seizure activity (status epilepticus) results in mitochondrial exhaustion and ATP depletion, which potentially generate energy mism...
ORGANISM(S): Rattus rattus (Black rat) 
2024-09-12 | PXD055090 | Pride
Epilepsy  metabolic adaptation  status epilepticus  atp production  shotgun proteomics 
ProteomicsCyclophilin A is not acetylated at Lysine-82 and Lysine-125 in resting and stimulated platelets
Cyclophilin A (CyPA), a member of the family of cyclophilins, is widely expressed by all prokaryotic and eukaryotic cells. Upon activation, CyPA can also be released from platelets, and engages in extracellular functions via interaction with the CD147 receptor that contribute to cardiovas...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-02-22 | PXD027289 | Pride
Acetylation Cyca Ptm 
ProteomicsHighly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography
The dataset contains the data of key experiments for developing a novel and highly sensitive bottom-up phosphoproteomics strategy termed ERLIC-SCX/RP-LC-MS. We compared two SPE types (RP, SCX) for an ERLIC run with 7 fractions. The results were used to design a workflow for highly sensitive bottom-u...
ORGANISM(S): Homo sapiens (Human) 
2014-12-02 | PXD001381 | Pride
Phosphoproteomics Phosphopeptide enrichment Lc-ms Solid-phase extraction Scx Protein phosphorylation Rp 
ProteomicsERLIC-based strategies for combined quantitative proteome and phosphoproteome analysis with limited sample amounts
The study aims the development of a sensitive strategy for combined quantitative proteomics and phosphoproteomics. 3 ERLIC-based strategies, applicable for low-sample-amount experiments (~150 µg per condition), were evaluated and compared . Three samle sets have been uploaded. 16 fractions ERLIC-1-S...
ORGANISM(S): Homo sapiens (Human) 
2015-02-25 | PXD001241 | Pride
Protein phosphorylation Erlic Quantitative proteomics Multidimensional separation 
ProteomicsNew alterations of the platelet proteome in type I Glanzmann thrombasthenia
The project contains raw and result files of a proteomics analysis of two patients with type 1 Glanzmann thrombasthenia (Patient1 and 2) caused by homozygous ITGA2b delG mutations and from family (heterozygous: Father1, Mother1, Sister1, healthy: Sister2) and unrelated controls. A label-free analysi...
ORGANISM(S): Homo sapiens (Human) 
2019-01-14 | PXD003912 | Pride
Glanzmann thrombasthenia Lc-ms Platelets Proteomics 
ProteomicsSemi-automated positive pressure FASP in 96-well format (PF96) enables highly reproducible and time-efficient proteomics sample preparation of larger cohorts
As novel technologies allow for a substantial increase in the number of LC-MS run per day, proteomics sample preparation appears as new bottle-neck for throughput. To overcome this limitation, we introduce a novel strategy for positive pressure 96-well filter-aided sample preparation (PF96) on a com...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2021-12-03 | PXD024236 | Pride
96-well format Proteomics Sample preparation Automation 
OtherHighly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography.
Data from ProteomeXchange, PXD ID: PXD001381. Experiment: ERLIC2, file: Velos02_06349 ERLIC2 F14.mzml. Published as part of Anal Chem. 2014 Nov 18 . From the Abstract: {{i}} ... Here we introduce a highly sensitive and easy-to-establish 2D bottom-up strategy for -g-scale phosphoproteomics, based on...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320005256 | GPMDB
Resource Reanalysis 
OtherMultidimensional electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) for quantitative analysis of the proteome and phosphoproteome in clinical and biomedical
Data from ProteomeXchange, PXD ID: PXD001241. Experiment: ERLIC-2 Rep2, file: folder summary. Published as part of Biochim Biophys Acta. 2015 S1570-9639(15)00017-5 . From the Abstract: {{i}} ... we generated a highly sensitive workflow for combined LC-MS-based quantitative proteomics and phosphopro...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32310007030 | GPMDB
Resource Reanalysis 
ProteomicsTowards zero variance in proteomics - positive pressure FASP in 96-well format (PF96) enables highly reproducible and time-efficient analysis of sample cohorts
As novel LC-MS technologies for proteomics offer substantial increase in LC-MS runs per day, robust and reproducible sample preparation emerges as a new bottleneck for throughput. We introduce a novel strategy for positive pressure 96-well filter-aided sample preparation (PF96) on a commercial posit...
ORGANISM(S): Mus Musculus 
2022-03-24 | PXD025089 | panorama
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