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ProteomicsThe quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells
Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs....
ORGANISM(S): Homo sapiens (Human) 
2013-02-01 | PXD000134 | Pride
Lc-msms Embryonic stem cells Reprogramming Fibroblast Etd Ips cells 
ProteomicsIntegrative genome, transcriptome and proteome analysis of rat livers from two different genetic backgrounds
In this project, we aim to pair-wise analyze the genomes, transcriptomes and proteomes of in-bred rats originating from two different genetic backgrounds. These two strains are Brown Norway (BN-Lx) and Spontaneously Hypertensive Rats (SHR). First, we re-sequenced the genomes for both BN and SHR rats...
ORGANISM(S): Rattus norvegicus (Rat) 
2013-11-29 | PXD000131 | Pride
Lc-msms Rat Proteome Livers 
OtherA systems-wide screen identifies substrates of the SCF-beta-TrCP ubiquitin ligase.
Data from ProteomeXchange, PXD ID: PXD001224. File: OR3_100623_BTrCP_EV_IP_Band01.mzml. Published as part of Sci Signal. 2014 Dec 16;7(356):rs8 . From the Abstract: {{i}} ... We developed a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for the system-wide iden...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320006530 | GPMDB
Resource Reanalysis 
ProteomicsCovalent Organic Framework with Acid Precipitation Enhances Microprotein Enrichment and Discovery of Ferroptosis-Associated Microproteins
Microproteins, defined as polypeptides fewer than 100 amino acids, are encoded by small open reading frames (sORFs) and exhibit unique structural and functional characteristics. Despite their short length and recent discoveries, microproteins have already been found to play critical roles in diverse...
ORGANISM(S): Homo Sapiens 
2026-02-26 | PXD074935 |
ProteomicsA High-resolution Systems-wide Screen for Substrates of the SCFβTrCP Ubiquitin Ligase - reproducibility evaluation
This project demonstrates a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for identifying in a systems-wide manner bone fide substrates of βTrCP
ORGANISM(S): Homo sapiens (Human) 
2015-01-06 | PXD001224 | Pride
Ap-ms Perseus Interaction proteomics Βtrcp Crapome Ubiquitin e3 ligase Fbw11 
ProteomicsA High-resolution Systems-wide Screen for Substrates of the SCFβTrCP Ubiquitin Ligase
This project demonstrates a combined bioinformatics and affinity purification-mass spectrometry (AP-MS) workflow for identifying in a systems-wide manner bone fide substrates of βTrCP
ORGANISM(S): Homo sapiens (Human) 
2015-01-06 | PXD001088 | Pride
Ap-ms Perseus Interaction proteomics Βtrcp Crapome Ubiquitin e3 ligase Fbw11 
ProteomicsAP-MS of different isoforms of Axin
This project aims to identify and differentiate the interaction partners of Axin1 isoforms including 1 wild type and 3 different mutants (L106R, L106R_F119R, deltaRGS)
ORGANISM(S): Homo sapiens (Human) 
2022-03-01 | PXD001822 | Pride
Ap-ms Wnt signaling Axin1 Proteomics Hek293 
ProteomicsAP-MS of tissue-specific protein complexes in C. elegans
We present a method for the tissue-specific purification of protein complexes from the nematode C. elegans, based on cell-type specific biotinylation. To purify proteins from specific C. elegans tissues, we adapted a system based on in vivo biotinylation of a protein of interest. In this approach, a...
ORGANISM(S): Caenorhabditis elegans 
2016-07-18 | PXD002139 | Pride
Bira biotin ligase C. elegans Ap-ms 
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