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ProteomicsTargeted Proteomics of NERD and FLS2 on LTQ-Orbitrap Velos Mass Spectrometer
Targeted proteomics has become increasingly popular recently because of its ability to precisely quantify selected proteins in complex cellular backgrounds. We demonstrate the utility of an LTQ-Orbitrap Velos Pro mass spectrometer in targeted parallel reaction monitoring (PRM) despite its unconventi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-12-09 | PXD001119 | Pride
Ltq-orbitrap Prm Fls2 Targeted proteomics Nerd 
MetabolomicsLipid Data Analyzer: The LDA algorithm against the in silico library LipidBlast (LipidBlast benchmarking experiment)
Lipid Data Analyzer (LDA; http://genome.tugraz.at/lda2) provides automated and reliable annotation of lipid species and their molecular structures in high-throughput data from chromatography-coupled tandem mass spectrometry using embedded rule sets. Using various low- and high-resolution mass spectr...
2017-11-07 | MTBLS397 | MetaboLights
ProteomicsOptimizing chromatographic conditions for orbitrap-based MS platforms
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
ORGANISM(S): Homo sapiens (Human) 
2019-03-04 | PXD009875 | Pride
Reproducibility Data-dependent acquisition Chromatography Q exactive Fusion lumos Human Pearson product-moment correlation coefficient Distributed normalized spectral abundance factor Orbitrap Quantitative proteomics Multidimensional protein identification technology 
ProteomicsGeLC-MS/MS analysis of hen egg vitelline membrane from freshly-laid eggs and eggs stored for 28 days at 4°C and 20°C
The hen egg vitelline membrane is a protein layer separating the yolk content from the egg white. The integrity of egg vitelline membrane is important for both the shell egg and the egg product industries. However, its physical properties are altered during prolonged storage, depending on storage te...
ORGANISM(S): Gallus gallus (Chicken) 
2026-03-16 | PXD066675 | Pride
Sds-page Egg Vitelline membrane Chicken Nanolc-ms/ms Storage 
ProteomicsNative Elongin A Co-IP on mouse embryonic stem cells nuclear extract using R19 goat EloA antibody
To identify proteins associated with mouse Elongin A (EloA), we carried out Co-IP on nuclear extract prepared from mouse ES cells from wild-type and null Elongin A cells (negative control).Each immunoprecipitation was excised as 5 gel sections, minced and digested in-gel using trypsin. Electroioniza...
ORGANISM(S): Mus Musculus (mouse) 
2020-12-10 | PXD022138 | JPOST Repository
ProteomicsDBR1 Interactors Identification Mass Spectrometry Analysis
DBR1 interactors were identified from HeLa-S cells.
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD042580 | Pride
Lc-msms Hela-s 
ProteomicsHarnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras
In this study, we identified the O-glycosylation sites in deletion constructs of recombinant Clostridium botulinum flagellin (CbFla) expressed from the E. coli strain, EV136 (K1:K12 strains engineered by Prof. Eric Vimr’s group to accumulate CMP-sialic acid in the cytosol). The recombinant flagellin...
ORGANISM(S): Clostridium botulinum str. LANGELAN F 
2024-08-22 | PXD044191 | Pride
Ltq orbitrap velos pro ion-trap mass spectrometer Nonulosonic acid O-glycosylation Neu5ac Sialic acid Kdo Clostridium botulinum Gram positive Flagellin Geobacillus kaustophilus Maf 
ProteomicsIdentification of O-glycosylation sites in Clostridium botulinum and Geobacillus kaustophilus flagellins
In this study, we identified the O-glycosylation sites in recombinant Clostridium botulinum flagellin (CbFla) and Geobacillus kaustophilus flagellins, GkFlaA1 and GkFlaA2, expressed from the E. coli strains, EV136 and EV240 (K1:K12 strains engineered by Prof. Eric Vimr’s group to accumulate CMP-sial...
ORGANISM(S): Clostridium botulinum F str. Langeland Geobacillus kaustophilus HTA426 
2020-09-07 | PXD018167 | Pride
Ltq orbitrap velos pro ion-trap mass spectrometer Nonulosonic acid O-glycosylation Neu5ac Sialic acid Kdo Clostridium botulinum Gram positive Flagellin Geobacillus kaustophilus Maf 
ProteomicsS-nitrosation of SCF E3 ligase components regulates hormonal signaling in Arabidopsis
E3 ubiquitin ligases mediate the last step of the ubiquitination pathwayin the ubiquitin-proteasome system (UPS). By targeting transcriptional regulators for their turnover, E3s play a crucial role in every aspect of plant biology. In plants, SKP1/CULLIN1/F-BOX PROTEIN (SCF)-type E3 ubiquitin ligase...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD030054 | Pride
S-nitrosation  hormonal signaling  arabidopsis 
ProteomicsMS data of tryptic peptides obtained through activity-guided protein purification
Protein band obtained from the Panax notoginseng leaves homogenate following an activity-guided protein-purification procedure, that showed regioselective ginsenoside hydrolysis activity was digested with trypsin and analyzed by Nano LC-LTQ Orbitrap MS to construct a peptide library of accessible am...
ORGANISM(S): Panax notoginseng 
2024-01-05 | PXD048221 | Pride
Tryptic peptides Orbitrap based lcms Panax notoginseng Plant glucosidase 
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