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UnknownDavid M. Lubman-The University of Michigan-A retrospective in research.
Not available
S-EPMC8903096 | biostudies-literature
ProteomicsCD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis
We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
Serum Immunoaffinity isolation Cd9 Extracellular vesicles Hplc Proteomics 
ProteomicsIntact N-glycopeptides in Serum Haptoglobin between HCC and Cirrhosis
A workflow for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis has been developed.
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD011239 | Pride
Human Serum Haptoglobin Lc-ethcd-ms/ms Liver 
ProteomicsGlycopeptide Biomarkers for HCC Detection in Patients with NASH
A comprehensive screening of site-specific N-glycopeptides in serum haptoglobin (Hp), a reporter molecule for aberrant glycosylation in HCC, has been performed to characterize glycopeptide markers for NASH-related HCCs.
ORGANISM(S): Homo sapiens (Human) 
2020-05-22 | PXD018502 | Pride
Human Serum Glycopeptide Lc-ethcd-ms/ms 
ProteomicsSingle amino acid variant
We performed deep proteomic profiling down to as few as 9 Panc-1 cells using sample fractionation, TMT multiplexing and carrier/reference strategy. Off line fractionation of the TMT-labeled sample pooled with TMT-labeled carrier Panc-1 whole cell proteome was achieved using alkaline reversed phase s...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD011471 | Pride
Human Panc-1 Lc-ms/ms 
Proteomicscomprehensive SAAVs detection in PANC-1 cell line
Three different strategies were applied for SAAVs detection. We predicted deleterious SAAVs using online AssVar and confirm 3 SAAVs using SRM
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD017449 | Pride
Saavs Panc-1 Srm Lc-ms/ms 
UnknownPancreatic Cancer Chemoprevention Translational Workshop: Meeting Report.
Not available
S-EPMC4988336 | biostudies-literature
ProteomicsIn-depth proteome profiling of small extracellular vesicles isolated from cancer cell lines and patient serum
Extracellular Vesicle (EV) secretion has been observed from most types of normal and tumor cells. These EVs contain a variety of distinctive cargo where tumor-derived serum proteins in EVs secreted into the bloodstream can be used to provide a minimally invasive method for clinical monitoring. We ha...
ORGANISM(S): Homo Sapiens (human) 
2024-09-08 | PXD045198 | JPOST Repository
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