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ProteomicsTandem LysC/trypsin digestion performs efficient in detergent conditions and under constant denaturating environments
All shotgun proteomics experiments rely on efficient proteolysis steps for sensitive peptide/protein identification and quantification. Continuous protocol improvement is therefore a constant topic in proteomics research. Previous reports suggest that the sequential tandem LysC/trypsin digest yields...
ORGANISM(S): Escherichia coli Bacteria 
2020-04-30 | PXD013273 | Pride
Shotgun proteomics Protein digestion Lysc Trypsin 
UnknownP2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC.
Not available
S-EPMC438596 | biostudies-literature
UnknownRole of lysine binding residues in the global folding of the lysC riboswitch.
Not available
S-EPMC4829333 | biostudies-literature
UnknownMolecular Characterization of a Novel Lytic Enzyme LysC from Clostridium intestinale URNW and Its Antibacterial Activity Mediated by Positively Charged N-Terminal Extension.
Not available
S-EPMC7404271 | biostudies-literature
ProteomicsConservation of mRNA and Protein Expression during Development of C.elegans
A detailed description of the sample processing is published (Grün et al., 2014 Cell Rep). Briefly, a super Silac Mix using Lys8 was generated and spiked into all samples as an internal reference. Total Lysate was fractionated using SDS-PAGES. Up to 16 fractions were collected per sample. Proteins w...
ORGANISM(S): Caenorhabditis elegans 
2015-07-09 | PXD002482 | Pride
Sds-page Lysc C.elegans 
UnknownmD-UPLC-MS/MS: Next Generation of mAb Characterization by Multidimensional Ultraperformance Liquid Chromatography-Mass Spectrometry and Parallel On-Column LysC and Trypsin Digestion.
Not available
S-EPMC9201819 | biostudies-literature
ProteomicsComparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows
This study presents a comparative analysis of three LysC endopeptidases homologues from Achromobacter lyticus, Pseudomonas aeruginosa, and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture (PAC) workflow with HeLa cell lysate, we assessed the enzy...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD068827 | Pride
Endoproteinase lys-c Proteomics workflow Method development Label-free quantitation Bottom-up proteomics Proteolytic enzymes Lc-ms/ms 
ProteomicsPTP1B treatment of phosphoproteome-derived GluC and LysC peptide libraries
One hour reactions of PTP1B treatment of phosphoproteome-derived peptide libraries made from pervanadate-treated HEK293T cells and digested with GluC or LysC
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2026-02-26 | MSV000100984 | MassIVE
ProteomicsMistranslating tRNA variants impact on the proteome and phosphoproteome of Saccharomyces cerevisiae – whole proteome DDA and DIA data
Mutations in tRNAs can lead to mis-incorporation of an amino acid into a growing polypeptide chain that differs from what is specified by the mRNA in a process known as mistranslation. As mistranslating tRNAs modify how the genetic message is decoded, they have potential as therapeutic tools to trea...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-11-18 | PXD068392 | Pride
Translation fidelity Trnas Saccharomyces cerevisiae Mistranslation Proteome Yeast 
ProteomicsWipA and WipB treatment of phosphoproteome-derived GluC and LysC peptide libraries
WipA or WipB treatment of phosphoproteome-derived peptide libraries made from pervanadate-treated HEK293T cells and digested with GluC or LysC.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2026-02-26 | MSV000100982 | MassIVE
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