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The Sertoli cells (Sc) of 5 days (infant) and 12 days (pubertal) old rat were isolated and cultured in triplicates. Nuclear and cytoplasmic fractionation was done for both the cases. All the four protein fractions (nuclear and cytoplasm of infant and pubertal Sc) were anaysed using Lc-MS/MS.
ORGANISM(S): Rattus norvegicus (Rat) 
2017-01-18 | PXD003050 | Pride
The Sertoli cells (Sc) of 5 days (infant) and 12 days (pubertal) old rat were isolated and cultured in triplicates. Nuclear and cytoplasmic fractionation was done for both the cases. All the four protein fractions (nuclear and cytoplasm of infant and pubertal Sc) were anaysed using Lc-MS/MS. SWATH a...
ORGANISM(S): Rattus norvegicus (Rat) 
2017-01-18 | PXD003056 | Pride
The project aimed to profile the cell surface proteins of Nomo-1 (AML cell line) using structural surfaceomics for identification of protein conformation-based cancer antigens thereby expanding the toolkit for cancer target discovery for immunotherapeutic targeting. To achieve the goal, cell surface...
ORGANISM(S): Homo sapiens (Human) 
2023-10-04 | PXD035589 | Pride
The project aimed to profile the cell surface proteins of Nomo-1 (AML cell line) using structural surfaceomics for identification of protein conformation-based cancer antigens thereby expanding the toolkit for cancer target discovery for immunotherapeutic targeting. To achieve the goal, cell surface...
ORGANISM(S): Homo sapiens (Human) 
2023-10-04 | PXD035591 | Pride
The goal was to build a custom proteome database for cell surface proteins of Nomo-1 (AML cell line). Such custom database was meant to facilitate cross-linking (XL-MS) of cell surface proteins for this cell line using non-cleavable cross-linkers. Using such custom database helps with the computatio...
ORGANISM(S): Homo sapiens (Human) 
2023-10-04 | PXD035404 | Pride
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