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ProteomicsE. coli BW25113 and knock-out mutants (delta-pka, delta-arcA and delta-pka_arcA) LC MS/MS
1ml of WT, delta-pka, delta-arcA and delta-pka delta-arcA culture broth was harvested at u=0.4 h-1, centrifuged (14000 x g for 1 min at 4 degrees C), pellet washed once with PBS, flash frozen with liquid N2 and kept at -80 degrees C until further processing. Frozen pellets were melted on ice after w...
ORGANISM(S): Escherichia coli Acyrthosiphon pisum (Pea aphid) 
2014-03-19 | PXD000556 | Pride
Delta-pka Delta-pka_arca Delta-arca E. coli 
ProteomicsE. coli BW25113 proteomics in nutrient-rich and minimal medium
E. coli BW25113 growth in a nutrient-rich medium supplemented with 20 proteinogenic amino acid medium was studied and compared to growth in minimal medium. Proteome data from these experiments revealed significant differences in amino acid synthesis and transport proteins that were reduced in the nu...
ORGANISM(S): Escherichia coli 
2020-02-18 | PXD011726 | Pride
E. coli rich medium 
ProteomicsThreonine concentration effect on L. lactis growth
L. lactis was grown in steady-state cultures at specific growth rate 0.2 h-1. The concentration of L-threonine in the growth environment was smoothly increased from 1.24mM to 4mM and proteome measurements were carried out to detect proteins involved in threonine metabolism.
ORGANISM(S): Lactococcus lactis 
2015-03-19 | PXD001602 | Pride
Lactococcus lactis Silac Threonine metabolism 
ProteomicsSpecific growth rate-dependent proteome resource allocation dynamics in Escherichia coli
Cells usually respond to changing growth conditions with a change in the specific growth rate (μ) and adjustment of their proteome to adapt and maintain metabolic efficiency. Description of the principles behind proteome resource allocation is thus important for understanding metabolic regulation in...
ORGANISM(S): Escherichia coli 
2015-02-27 | PXD001594 | Pride
E. coli label-free specific growth rate dependent data 
UnknownThe effects of nalmefene on the impulsive and reflective system in alcohol use disorder: A resting-state fMRI study.
Not available
S-EPMC9293828 | biostudies-literature
ProteomicsThe Novel PII-Interacting Regulator PirC (Sll0944) Identifies 3-Phosphoglycerate Mutase (PGAM) as Central Control Point of Carbon Storage Metabolism in Cyanobacteria
Nitrogen limitation imposes a major transition in the life-style of non-diazotrophic cyanobacteria, which is regulated via a complex interplay of regulatory factors, involving, the nitrogen-specific transcription factor NtcA and the pervasive signal processor PII. Immediately upon nitrogen-limitatio...
ORGANISM(S): Synechocystis sp. PCC 6803 
2020-12-17 | PXD021415 | Pride
Pirc (sll0944) Cyanobacteria Synechocystis sp. pcc 6803 3-phosphoglycerate mutase Pii 
UnknownNalmefene attenuates neural alcohol cue-reactivity in the ventral striatum and subjective alcohol craving in patients with alcohol use disorder.
Not available
S-EPMC8292278 | biostudies-literature
ProteomicsDisruption of the human Cystin-1 myristoyl-electrostatic switch causes autosomal recessive polycystic kidney disease (ARPKD)
Autosomal recessive polycystic kidney disease (ARPKD; MIM#263200) is primarily caused by pathogenic variants in PKHD1, which encodes fibrocystin/polyductin (FPC). The Cys1cpk/cpk (cpk) mouse expresses a renal lesion that closely phenocopies ARPKD. Cys1-encoded cystin is a myristoylated protein that ...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-13 | PXD048013 | Pride
Arpkd Myristoyl-electrostatic switch Pka-phosphorylation Ppm1a-dephosphorylation Cystin Cystin-1 
ProteomicsThe signal transduction protein PII controls ammonium, nitrate and urea uptake in cyanobacteria
PII signal transduction proteins are widely spread among all domains of life where they regulate a multitude of carbon and nitrogen metabolism related processes. Non-diazotrophic cyanobacteria can utilize a high variety of organic and inorganic nitrogen sources. In recent years, several physiologica...
ORGANISM(S): Synechocystis sp. PCC 6803 
2019-07-10 | PXD013411 | Pride
Urea uptake Nitrogen regulation Abc transporters Nitrate uptake Ammonium uptake Glnb Cyanobacteria Pii signaling protein 
UnknownDurvalumab, with or without tremelimumab, plus platinum-etoposide in first-line treatment of extensive-stage small-cell lung cancer: 3-year overall survival update from CASPIAN.
Not available
S-EPMC9161394 | biostudies-literature
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