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Streptococcus agalactiae strain:mast1 | isolate:mast1 Genome sequencing
A complex of MAST1 and 14-3-3η was co-expressed and co-purified from Sf9 insect cells. Since the interaction between MAST1 and 14-3-3 is dependent on phosphorylation of MAST1, the complex was analyzed by tandem mass spectrometry to determine which MAST1 residues are phosphorylated.
ORGANISM(S): Mus musculus (Mouse) 
2026-09-02 | PXD063795 | Pride
MAST1 was N-terminally EGFP-tagged and overexpressed in HEK293 cells. Two constructs of MAST1, (WT and S90A S161A), as well as an EGFP only control, were compared in a pairwise manner to identify specific interactors of MAST1. EGFP-MAST1 was affinity-purified using the GFP-Trap system and then incub...
ORGANISM(S): Homo sapiens (Human) 
2026-09-02 | PXD080824 | Pride
Purified MAST1, spanning residues 116-724, underwent autophosphorylation in vitro. The resultant phosphosites on MAST1 were mapped using tandem mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2026-09-02 | PXD063802 | Pride
Purified MAST1, spanning residues 70-350, was phosphorylated by purified full length PAK4 in vitro. The resultant phosphosites on MAST1 were mapped using tandem mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2026-09-02 | PXD063803 | Pride
MAST1 was N-terminally EGFP-tagged and overexpressed in HEK293 cells. Various constructs of MAST1, including WT, S90A, S161A, S90A S161A, and N-terminal truncations spanning S90 and S161 were compared in a pairwise manner to identify specific interactors of MAST1. EGFP-MAST1 was immunoprecipitated u...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-09-02 | PXD063727 | Pride
Purified full-length Tau was phosphorylated in vitro by purified MAST1 (116-724). The resultant phosphosites on Tau were mapped using tandem mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2026-09-02 | PXD063731 | Pride
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