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ProteomicsNovel PPT+SEC Workflow for High-Sensitivity Extracellular Vesicle Proteomics from Cell Media
Size exclusion chromatography (SEC) is a well-established method for the isolation of extracellular vesicles (EVs), but the large elution volumes necessitate a concentration step prior to proteomics analysis. This concentration step can lead to significant EV loss. Here we report an EV proteomics ap...
ORGANISM(S): Homo sapiens (Human) 
2025-05-03 | PXD060798 | Pride
Micro proteomics Extracellular vesicles Pancreatic cancer 
ProteomicsInvestigation of region specific lipid alterations in symptomatic atherosclerotic plaques using mass spectrometry imaging
Atherosclerotic plaques are composed by different cell populations that interact one with each other in a complex and heterogeneous environment. Here we report a MALDI mass spectrometry imaging investigation of the lipid profile of specific plaque regions, to discriminate between symptomatic and asy...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023663 | Pride
Plaque vulnerability Macrophages Maldi msi Lipids Atherosclerosis Vsmcs 
ProteomicsLow-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics
We report an investigation of low-temperature embedding media in terms of their mechanical properties and embedding temperature, with the goal of determining a reliable embedding medium for the combination of MALDI MSI of lipids, laser-capture microdissection of tissue regions, and quantitative prot...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD045349 | Pride
Proteomics Laser-capture microdissection Maldi msi Embedding media 
ProteomicsCandidate early biomarkers of Glioblastoma Multiforme revealed by longitudinal bottom-up proteomics study on multiple biofluids in a murine model
Glioblastoma Multiforme (GBM) is an aggressive form of brain tumor, associated with poor prognosis and low survival rates, and early diagnosis is impaired by the lack of presymptomatic biomarkers. Here we used a syngenic GBM mouse model to investigate longitudinal changes in the proteome profile of ...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD020285 | Pride
Proteomics Extracellular vesicles Glioblastoma Serum Csf. Biomarkers Longitudinal 
ProteomicsMetabolic Trajectories in Human Neocortical Development
The shift from glycolysis to oxidative phosphorylation is a key step in neural differentiation. Yet, the timing of metabolic rewiring in the development from progenitors to neurons remains elusive, especially in human corticogenesis. To bridge this gap, here we adopted a unique cell platform based o...
ORGANISM(S): Homo sapiens (Human) 
2026-03-18 | PXD058952 | Pride
Neurons Neocortex Metabolism Neuroepithelial stem cells 
ProteomicsRegion-specific lipid markers of plaque outcome revealed by mass spectrometry imaging of human carotid atherosclerotic plaques
The goal of the project is the detection of region-specific lipid features that discriminate between symptomatic and asymptomatic human carotid atherosclerotic plaques by MALDI MSI.
ORGANISM(S): Homo sapiens (Human) 
2025-01-04 | PXD039822 | Pride
Atherosclerosis Maldi msi Lipids Plaque outcome 
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