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ProteomicsF1 allele specific gene expression
By comparing mouse fibroblasts from two parental strains (Bl6 and Spretus) with fibroblasts from their first generation offspring (F1) we can detect allele specific expression of proteins. The Bl6 and Spretus lines are evolutionary distant and harbour many SNPs in their genomes which when synonomous...
ORGANISM(S): Mus musculus (Mouse) 
2015-08-18 | PXD002337 | Pride
Lc-ms/ms Bl6 Azidohomoalanine Silac F1 Spretus Allele Monolithic column 
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo sapiens (Human) 
2016-10-06 | PXD004915 | Pride
Human Aneuploidy Pre-1 
ProteomicsNIH 3T3 AHA p-c, SILAC p-c, inhibitor, and enrichment control experiments
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. These experiments were performed with 0, 1, 2, 4, 8, 16 and 13 h of chase. Data from 3 biological replicates ar...
ORGANISM(S): Mus musculus (Mouse) 
2016-10-06 | PXD004929 | Pride
Azidohomoalanine Mouse Silac Degradation Aneuploidy 
ProteomicsQuaNCAT analysis comparing proteins synthesized in neurites and soma in ASCL1-iNeurons
Local translation in ASCL1-iNeurons was probed using QuaNCAT
ORGANISM(S): Mus musculus (Mouse) 
2018-10-17 | PXD005059 | Pride
Azidohomoalanine Mouse Silac 
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080831 | MassIVE
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