Sort   by:  
 Page size 
ProteomicsHistone dynamics at replication forks in embryonic stem cells
We employ inducible mutants to dissect the early and late consequences of impaired histone recycling. We find rapid accumulation of H3K27me3 in response to impaired histone recycling, preceding gene expression changes. Simultaneous disruption of both leading (POLE4) and lagging strand (MCM2-2A) recy...
ORGANISM(S): Mus musculus (Mouse) 
2025-01-12 | PXD053300 | Pride
Parental histones Pole4 Mcm2 Escs 
ProteomicsQuantification of parental and new histones H3, H4 and H2A by mass spectrometry: analysis of their dynamics along cell cycle progression in WT and MCM2-2A mutant mouse ESCs
During DNA replication parental, nucleosomic histones are segregated almost symmetrically to the two daughter DNA strands enabling mitotic inheritance of histones and their PTMs. In MCM2 histone-binding mutant ESCs (MCM2-2A), histones are recycled asymmetrically to the leading strand. To evaluate if...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-30 | PXD030364 | Pride
Histone Mouse Ms Silac Pulse-silac Esc Mcm2 
ProteomicsProximal proteomics analysis reveals DNA polymerase δ subunit 3 is a new MCM2 binding partner and promotes
Our TurboID proximity labeling proteomics approach describes a comprehensive analysis of the histone recycling network.
ORGANISM(S): Mus musculus (Mouse) 
2025-12-01 | PXD048030 | Pride
TranscriptomicsMcm2 promotes stem cell differentiation via its ability to bind H3-H4 (RNA-Seq)
Mcm2, a subunit of the Mcm2-7 helicase best known for its role in DNA replication, contains a histone binding motif that facilitates the transfer of parental histone following DNA replication. Here we show that Mcm2 is important for the differentiation of mouse embryonic stem (ES) cells. Mcm2-2A mut...
ORGANISM(S): Mus musculus 
2022-11-16 | GSE203271 | GEO
GenomicsMcm2 promotes stem cell differentiation via its ability to bind H3-H4 (ChIP-Seq)
Mcm2, a subunit of the Mcm2-7 helicase best known for its role in DNA replication, contains a histone binding motif that facilitates the transfer of parental histone following DNA replication. Here we show that Mcm2 is important for the differentiation of mouse embryonic stem (ES) cells. Mcm2-2A mut...
ORGANISM(S): Mus musculus 
2022-11-16 | GSE203270 | GEO
ProteomicsQuantification of histone H3 and H4 modifications by mass spectrometry in wildtype and MCM2-2A mutants of mice ESCs
During DNA replication modified parental histones H3-H4 are segregated almost symmetrically to the two daughter DNA strands enabling mitotic inheritance of histone PTMs. Whether heritable histone modifications confer epigenetic regulation by maintaining chromatin states and gene expression is unclea...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-30 | PXD020326 | Pride
H3 acetylation H4 methylation Histones H3 methylation Escs Mcm2 Mcm2-2a 
TranscriptomicsProper parental histone allocation is required for mouse embryonic stem cell differentiation and histone post-transcription modification inheritance
To understand whether parental histone PTM landscape plays roles in cell differentiation, we perturbed parental histone PTM allocation by mutating histone chaperone MCM2 on its histone binding domain (MCM2-2A mutation), disrupted only the histone transferring of MCM2 protein in mouse embryonic stem ...
ORGANISM(S): Mus musculus 
2023-05-15 | GSE185271 | GEO
GenomicsSymmetric inheritance of parental histones governs epigenome maintenance and stem cell identity [ChIP-seq]
Modified parental histones are segregated symmetrically to daughter DNA strands during replication and inherited through mitosis. How this may sustain the epigenome and cell identity remains unknown. Here, we show that transmission of histone-based information during replication maintains epigenome ...
ORGANISM(S): Mus musculus 
2023-06-15 | GSE154379 | GEO
TranscriptomicsSymmetric inheritance of parental histones governs epigenome maintenance and stem cell identity
Modified parental histones are segregated symmetrically to daughter DNA strands during replication and inherited through mitosis. How this may sustain the epigenome and cell identity remains unknown. Here, we show that transmission of histone-based information during replication maintains epigenome ...
ORGANISM(S): Mus musculus 
2023-06-15 | GSE154391 | GEO
TranscriptomicsSymmetric inheritance of parental histones governs epigenome maintenance and stem cell identity [RNA-seq]
Modified parental histones are segregated symmetrically to daughter DNA strands during replication and inherited through mitosis. How this may sustain the epigenome and cell identity remains unknown. Here, we show that transmission of histone-based information during replication maintains epigenome ...
ORGANISM(S): Mus musculus 
2023-06-15 | GSE154390 | GEO
Sort   by:  
 Page size