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ProteomicsCharmeRT - CharmeRT: Boosting peptide identifications by chimeric spectra identification and retention time prediction
Files include several HeLa tryptic digests with different isolation widths and different gradient times for chimeric spectra identification and validation, each of them as three technical replicates
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-06-09 | MSV000089624 | MassIVE
ProteomicsRobust and easy-to-use one pot workflow for label free single cell proteomics
The analysis of ultra-low input samples or even individual cells is essential to answering a multitude of biomedical questions, but current proteomic workflows are limited in their sensitivity and reproducibility. Here we report a comprehensive workflow that includes optimized strategies for all ste...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-01-26 | MSV000091156 | MassIVE
ProteomicsCharmeRT - CharmeRT: Boosting peptide identifications by chimeric spectra identification and retention time prediction
Files include several HeLa tryptic digests with different isolation widths and different gradient times for chimeric spectra identification and validation, each of them as three technical replicates
ORGANISM(S): Homo sapiens (Human) 
2018-06-12 | PXD007750 | Pride
Chimeric spectra Hela Lc-msms 
ProteomicsChasing protein arginine phosphorylation: development of a selective enrichment method using a trapping mutant phosphatase
Proof-of-principle pull-down experiments using a YwlE trapping mutant phosphatase to selective capture arginine phosphorylated proteins. Phosphoarginine is an acid labile protein modification that was demonstrated to exist in bacteria and probably also in higher eukaryotes. Due to the high abundance...
ORGANISM(S): Bacteria Bacillus subtilis subsp. subtilis str. 168 
2014-05-19 | PXD000560 | Pride
Arginie phosphorylation Trapping mutant phosphatase 
ProteomicsBacillus subtilis ClpP degradome
To characterize the proteins substrates and the modifications of the ClpP protease of Bacillus subtilis.
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2016-10-10 | PXD003305 | Pride
Clpp Protein degradation Phosphoarginine 
ProteomicsComprehensive XL-MS reveals parallel orientation and versatile conformations of plant HOP2/MND1
The HOP2/MND1 heterodimer is essential for meiotic homologous recombination in plants and other eukaryotes, and promotes the repair of DNA double strand breaks. The HOP2/MND1 dimer forms via the central, split coiled coils (CC1 and CC2) present in both proteins, and the resulting complex contains se...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-11-05 | PXD001538 | Pride
Xl hop2 mnd1 cross link 
ProteomicsImproved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Nano-lc-esi-ms/ms nano-flow hplc hela cell digest 
Proteomics15 cm Aurora Gen3 column benchmarking on an Exploris 480
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) 
2024-02-13 | PXD045457 | Pride
Vanquish neo Orbitrap exploris 480 Trap-and-elute 
ProteomicsLabel-Free Single Cell Proteomics Utilizing Ultrafast LC and MS Instrumentation: A Valuable Complementary Technique to Multiplexing.
In recent years, single cell proteomics became one of the hottest topics within the field of proteomics. The potential ability to map the proteomic fingerprint to transcriptomic data would master the understanding of how gene expression translates into actual phenotypes and cellular functions. In co...
ORGANISM(S): Homo sapiens (Human) 
2023-02-09 | PXD039208 | Pride
Dia Label-free-proteomics Data-completness Dda 
ProteomicsBenchmarking of 5.5 cm, 50 cm and 110 cm micropillar array columns as compared to packed bed columns
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD039576 | Pride
E. coli Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Trap-and-elute 
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