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Immuno-precipitation followed by MS was performed using the RIME protocol in this study. Estrogen Receptor (ER) and Progestorone Receptor (PR) were targetted using antibodies. The experiments were performed in a quantitative manner using SILAC labelling. Cells grown in complete serum (estrogenic) co...
ORGANISM(S): Homo sapiens (Human) 
2015-05-26 | PXD002104 | Pride
Estrogen receptor-alpha (ER) is the driving transcription factor in most breast cancers, and its associated proteins can influence drug response, but direct methods for identifying interacting proteins have been limited. We purified endogenous ER using an approach termed RIME (rapid immunoprecipitat...
ORGANISM(S): Homo sapiens (Human) 
2013-08-09 | PXD000047 | Pride
This project contains the raw and result files for a mass spectrometry analysis comparing the abundance of proteins following chromatin fractionation in growing cells and in senescent cells for the WI-38hTERT/GFP-RAF1-ER cell line. Two replicas were analysed for each cell condition. The data are use...
ORGANISM(S): Homo sapiens (Human) 
2019-09-25 | PXD014929 | Pride
Data from ProteomeXchange, PXD ID: PXD002104. File: PR333_HIM_020713_JP550_T47D_ER_R5020.mzml. From ProteomeXchange: {{i}} Immuno-precipitation followed by MS was performed using the RIME protocol in this study. Estrogen Receptor (ER) and Progestorone Receptor (PR) were targeted using antibodies. Th...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
This submission contains the mass spectrometry files for the manuscript by Uuskula-Reimand et al. that investigates the interactome of TOP2B using BioID and its genomic binding profile. This submission contains 18 IDA files for the following baits: empty 3XFLAG control, GFP-BirA-FLAG control, NLS-Bi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-07-10 | MSV000079188 | MassIVE
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