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ProteomicsAn improved capillary isoelectric focusing-mass spectrometry method for characterization of monoclonal antibody charge variants
Here, we improved the stability and enhanced separation resolution of automated cIEF-MS by bettering the quality of capillary neutral coating, reducing catholyte pH to 10 for cIEF-MS for the first time, and systematically optimizing the cIEF separation conditions for characterization of monoclonal a...
ORGANISM(S): Homo sapiens (Human) 
2026-08-19 | PXD082799 | Pride
Monoclonal antibody charge variants Cief-ms 
ProteomicsCharacterization of Monoclonal Antibody Charge Variants under Near-Native Separation Conditions using Nanoflow Sheath Liquid Capillary Electrophoresis-Mass Spectrometry
Monoclonal antibodies (mAbs) can undergo post-translational modifications (PTMs) in the production process, these are part of the critical quality attributes (CQA) to ensure protein safety. Charge variants are important PTMs in mAbs and can be separated by using capillary zone electrophoresis (CZE)....
ORGANISM(S): Monoclonal Antibodies 
2024-04-12 | MSV000094521 | MassIVE
ProteomicsDilute-and-Shoot Analysis of Therapeutic Monoclonal Antibody Variants in Fermentation Broth: A Method Capability Study
Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient thera-peutic agents for various diseases, including cancer, inflammatory and autoimmune diseases. As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs r...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD011017 | Pride
Glycation Monoclonal antibodies Process analytical technology Electrospray ionization mass spectrometry Pat Glycosylation Biosimilar Mam Multiple attribute monitoring Antibody subunits Structural analysis Fermentation Process monitoring High-performance liquid chromatography 
TranscriptomicsGene expression profiles of Herceptin-resistant breast cancer cells
Herceptin (trastuzumab) is a humanized monoclonal antibody targeted to the Her2 receptor tyrosine kinase. Despite a robust response rate to Herceptin-based therapies in Her2-positive patients, resistance frequently arises within one year of the initial response. To address the mechanism of Hercept...
ORGANISM(S): Homo sapiens 
2009-08-21 | E-GEOD-15043 | biostudies-arrayexpress
UnknownConformational characterization of the charge variants of a human IgG1 monoclonal antibody using H/D exchange mass spectrometry.
Not available
S-EPMC3564876 | biostudies-literature
UnknownCharge variant native mass spectrometry benefits mass precision and dynamic range of monoclonal antibody intact mass analysis.
Not available
S-EPMC6284562 | biostudies-literature
UnknownDesign and optimization of membrane chromatography for monoclonal antibody charge variant separation.
Not available
S-EPMC10078440 | biostudies-literature
UnknownEvaluation of a cIEF Fractionation Workflow for Offline MS Analysis of Charge Variants of the Monoclonal Antibody Matuzumab.
Not available
S-EPMC11865688 | biostudies-literature
UnknownImproved process intermediate stability through the identification and elimination of reactive glycation residues - a monoclonal antibody case study.
Not available
S-EPMC9342189 | biostudies-literature
UnknownRole of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates.
Not available
S-EPMC7512024 | biostudies-literature
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