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GenomicsEscherichia coli str. K-12 substr. MG1655
Identification of RNAs bound to MS2 aptamer using MS2-affinity purification coupled with RNA sequencing (MAPS)
PRJNA280419 | ENA
ProteomicsAffinity chromatography-based identification of sRNA protein partners in Rhizobia
This project aims to identify novel protein partners of conserved trans-sRNAs in the α-proteobacterium Sinorhizobium meliloti. Trans-sRNAs (AbcR2, NfeR1 and EcpR1) were tagged at their 5’ ends with the MS2 aptamer. To identify the proteins associated with the trans-sRNAs we: i) expressed and purifie...
ORGANISM(S): Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) 
2020-10-19 | PXD006410 | Pride
Trans-srnas Ms2 aptamer Sinorhizobium meliloti Lc-ms/ms 
ProteomicsE. coli Affinity purification MS/MS
The tRNA linked repeat sequence rrB was tagged in either 5'- or 3'- end with the MS2 aptamer and expressed from a plasmid in E. coli. Using the MS2 aptamer, the RNAs were affinity purified after cell lysis. The co-purifying proteins were identified by NanoLC-MS/MS and compared to the proteins co-pur...
ORGANISM(S): Escherichia coli 
2021-04-26 | PXD013749 | Pride
Ms2 Affinity purification Tlr Coli Rna 
ProteomicsAptamer-conjugated gold nanoparticles enable selective oligonucleotide delivery into muscle 2 stem cells to promote regeneration of dystrophic muscles
Inefficient targeting of muscle stem cells (MuSCs) represents a major bottleneck of current 27 therapeutic strategies for muscular dystrophies, as it precludes the possibility to promote 28 compensatory regeneration. Here we describe a novel delivery platform, based on gold nanoparticles, 29 that en...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-11 | PXD049295 | Pride
Gold nanoparticles Selex Proteomics Oligonucleotide Duchenne muscular dystrophy Aptamer 
UnknownIn vivo expression and purification of aptamer-tagged small RNA regulators.
Not available
S-EPMC2777422 | biostudies-literature
ProteomicsUsing MS2 affinity pulldown to identify sRNA associating proteins in the bacterium Deinococcus radiodurans
We carried out the widely used MS2 aptamer approach to identify the proteins that could potentially interact with sRNAs in D. radiodurans. The MS2 aptamer was fused to the 5 extremity of PprS/Dsr2, a well-characterized D. radiodurans sRNA . This construct is then expressed off the pRADgro plasmid in...
ORGANISM(S): Deinococcus Radiodurans R1 (ncbitaxon:243230) 
2024-05-23 | MSV000094854 | MassIVE
UnknownThe crystal structure of a high affinity RNA stem-loop complexed with the bacteriophage MS2 capsid: further challenges in the modeling of ligand-RNA interactions.
Not available
S-EPMC1370665 | biostudies-literature
GenomicsSinorhizobium meliloti
The mRNA interactomes of the homologous Sinorhizobium meliloti trans-sRNAs AbcR1 and AbcR2 determined by MS2-affinity purification coupled with RNA sequencing (MAPS).
PRJNA735891 | ENA
TranscriptomicsIdentification of RNAs bound to MS2 aptamer using MS2-affinity purification coupled with RNA sequencing (MAPS)
DsrA regulatory RNA represses both hns and rbsD mRNAs through distinct mechanisms in Escherichia coli
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2015-07-16 | GSE67606 | GEO
UnknownAn RNA-aptamer-based two-color CRISPR labeling system.
Not available
S-EPMC4882555 | biostudies-literature
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