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TranscriptomicsHT-SELEX of recombinant C2H2 zinc finger domains of Sall4
To define the sequence preference of SALL4 C2H2 zinc finger domains, we performed SELEX coupled with high-throughput sequencing (HT-SELEX) using the purified SALL4 ZFC1, ZFC2 and ZFC4 domains combined with no protein control experiment.
ORGANISM(S): artificial sequences 
2021-01-05 | E-MTAB-9236 | biostudies-arrayexpress
GenomicsAn improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-M-NM-:B as an example
We provided an improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor. We valided the strategy by characterzing the DNA-binding specificty of NF-M-NM-:B p50 dimer. Proteins of the Nf-kappab family were bound to DNA oligonucleotides containing a degenerate regi...
ORGANISM(S): Homo sapiens 
2014-05-05 | E-GEOD-48660 | biostudies-arrayexpress
GenomicsHT-SELEX of recombinant C2H2 zinc finger domains of Sall4
To define the sequence preference of SALL4 C2H2 zinc finger domains, we performed SELEX coupled with high-throughput sequencing (HT-SELEX) using the purified SALL4 ZFC1, ZFC2 and ZFC4 domains combined with no protein control experiment. We re-sequenced the libraries from E-MTAB-9236 with very high c...
ORGANISM(S): synthetic construct 
2022-03-01 | E-MTAB-11484 | biostudies-arrayexpress
GenomicsHT-SELEX of wild-type and mutated versions of recombinant DNA binding domain of SALL4
To investigate the differences in DNA binding specificity of wild-type SALL4 C2H2 zinc finger cluster 4 (ZFC4) and disease causing mutations in SALL4 ZFC4, we performed SELEX coupled with high-throughput sequencing (HT-SELEX) using the purified wild-type SALL4 ZFC4 domain, mutated SALL4 ZFC4 (R900W)...
ORGANISM(S): synthetic construct 
2023-01-04 | E-MTAB-11519 | biostudies-arrayexpress
GenomicsQuantitative modeling of transcription factor binding specificities using DNA shape
The SELEX-seq platform was used to generate DNA-binding affinity predictions for the human Max transcription factor. This experiment was performed as part of a cross-validation study comparing the accuracy of DNA shape-augmented TF binding specificity models across two different platforms (SELEX-se...
ORGANISM(S): Homo sapiens 
2015-03-01 | E-GEOD-60200 | biostudies-arrayexpress
GenomicsThe SET-domain protein SUVR5 mediates H3K9me2 deposition and silencing at stimulus response genes in a DNA methylation independent manner [SELEX]
In eukaryotic cells, environmental and developmental signals alter chromatin structure and modulate gene expression. Heterochromatin constitutes the transcriptionally inactive state of the genome and in plants and mammals is generally characterized by DNA methylation and histone modifications such a...
ORGANISM(S): Arabidopsis thaliana 
2014-06-06 | E-GEOD-58034 | biostudies-arrayexpress
GenomicsSelection of RNA aptamers against human islets by SELEX and toggle SELEX
Selection of RNA aptamers against human islets by SELEX and toggle SELEX
PRJNA809588 | ENA
GenomicsSELEX-seq of AP1 homodimer
SELEX-seq of AP1 homodimer
PRJNA957781 | ENA
Genomicscell-SELEX-AnnexinA2
Cell-SELEX identify an aptamer named ACE4 against the Annexin A2
PRJEB22637 | ENA
OtherSelection of RNA aptamers against human islets by SELEX and toggle SELEX
Type 1 Diabetes is still an incurable disease characterized by autoimmune destruction of insulin-producing beta cells within the islet of Langerhans in the pancreas. Currently, there are no methods to monitor beta-cell mass in humans or deliver therapeutics specifically to beta cells. Here we perfor...
ORGANISM(S): synthetic construct 
2022-02-23 | GSE197262 | GEO
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