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GenomicsQuantification of differential gene expression by multiplexed targeted resequencing of cDNA
Quantification of differential gene expression by multiplexed targeted resequencing of cDNA
PRJNA374355 | ENA
ProteomicsTMTPro Complementary Ion Quantification Increase Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 level
The ability of the TMTPro isobaric labeling reagents to form complementary ions for accurate multiplexed proteomics at the MS2 level was investigated. Human and yeast peptides were labeled in distinct ratios to analyze the effect of interference on the quantification accuracy. A method, TMTProC, was...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2021-05-11 | PXD021661 | Pride
Complementary ion quantification Interference-free quantification Multiplexing Tmtpro 
Proteomics102-Plex Approach for Accurate and Multiplexed Proteome Quantification
Numerous hyperplexing approaches have been developed to meet the demand for large-scale basic and clinical analysis. Currently, the analysis capacity has expanded to up to 54 samples within a single experiment by utilizing different isotopic and isobaric reagent combinations. In this report, we prop...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-01-15 | PXD042398 | Pride
Multiplexing Quantitative proteomic analysis 102-plex approach 
ProteomicsMultiplexed temporal quantification of the human plasma peptidome
The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and post-translational modifications and is a rich source of biomarkers and novel bioactives. To examine the utility of peptidomics we have examined the effects o...
ORGANISM(S): Homo sapiens (Human) 
2017-11-21 | PXD004781 | Pride
Peptidomics Exercise Peptidome Plasma 
ProteomicsAc-IP tag enables multiplexed proteome quantification in data-independent acquisition mode
We propose the isotopically labeled Ac-IP (acetyl-isoleucine-proline) tag for multiplexed quantification of peptides at the MS1 level in the data-independent acquisition (DIA) mode. Differentially labeled peptides have distinct precursor ions carrying the quantitative information but identical MS2 s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-07 | PXD022606 | Pride
Quantitative proteomics Isotope labeling Data-independent acquisition 
TranscriptomicsQuantification of differential gene expression by multiplexed targeted resequencing of cDNA
We present an approach for quantification of differential mRNA expression by targeted resequencing of cDNA using single-molecule molecular inversion probes (cDNA-smMIPs), which enable highly multiplexed resequencing of cDNA target regions of ~100 nt and counting of individual molecules. We show that...
ORGANISM(S): synthetic construct Homo sapiens 
2017-03-01 | GSE94800 | GEO
ProteomicsMaximally-multiplexed proteome quantification platform for isotopic metabolic and chemical labeling
Quantitative proteomic platforms based on precursor intensity in mass spectrometry (MS1-level) uniquely support in vivo metabolic labeling with superior quantification accuracy but suffers from limited multiplexity (≤3-plex) and frequent missing quantities. Herein, we present a maximally-multiplexed...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD009952 | Pride
Diethylation Heat shock Silac-6plex Protein quantification Hela cell Quantification Silac De-6plex Heat shock response 
ProteomicsEASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
We developed EASI-tag (Easily Abstractable Sulfoxide-based Isobaric tag), a new generation of amine-derivatizing and sulfoxide-containing isobaric labelling reagents, which dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient iso...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-06-21 | PXD007165 | Pride
Isobaric labeling Proteomics Mass spectrometry 
UnknownNeucode Labels for Multiplexed, Absolute Protein Quantification.
Not available
S-EPMC5141612 | biostudies-literature
ProteomicsMEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATS for Large Scale Absolute Proteome Quantification C4PR_LIV
A major challenge in proteomics is the absolute accurate quantification of large numbers of proteins. QconCATs, artificial proteins that are concatenations of multiple standard peptides, are well established as an efficient means to generate standards for proteome quantification. Previously, QconCAT...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-10-27 | PXD007949 | Pride
Qconcat meercat 
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