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ProteomicsHydrophobic tagging-assisted N-termini enrichment (HYTANE) for in-depth N-terminome analysis
The analysis of protein N-termini is of great importance for understanding the protein function and elucidating the proteolytic processing. Herein, we develop a negative enrichment strategy, termed as hydrophobic tagging-assisted N-termini enrichment (HYTANE) to achieve a global N-terminome analysis...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2016-08-19 | PXD004690 | Pride
Sacchoromyces cerevisiae Jurkat N-termini Caspase 
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana leaves using different proteases
Comparison of protein N-termini in Arabidopsis thaliana leaves by the HUNTER method; the corresponding digestion was performed with legumain, GluC and trypsin.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-20 | PXD014697 | Pride
Arabidopsis hunter n-termini profiling 
GenomicsHigh-throughput pyrosequencing of forum domains termini (FT) isolated and amplified from human HEK293T cells
Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Mostly forum domains are of 50-200 kb in length, although larger domains, up to 500 - 700 kb, are also observed. We performed a genome-wide mapping of forum ...
ORGANISM(S): Homo sapiens 
2013-03-31 | E-GEOD-35065 | biostudies-arrayexpress
ProteomicsN-terminome profiling in HEK293T
Various forms of the protein, called proteoform, are generated by co- or post-translational modification, alternative splicing and alternative translation initiation site, resulting in interactions between ribosomes, mRNAs, tRNAs, and various enzymes. Here, we introduce a N-terminal peptide enrichme...
ORGANISM(S): Homo sapiens (Human) 
2017-08-17 | PXD005583 | Pride
Protein stability Alternative translational initiation site Acetylation N-terminome Positional proteomics Proteoform N-termini Fasp N-end rule Translational initiation site 
OtherAnnotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome.
Data from ProteomeXchange, PXD ID: PXD000434. Experiment: 111213_RBC5_MG, file: 111213_RBC5_MG_Fr15_17.mzml. Published as part of J Proteome Res. 2014 Apr 4;13(4):2028-44 . From the Abstract: {{i}} Enucleated and largely void of internal membranes and organelles, erythrocytes are simple yet proteom...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310000272 | GPMDB
Resource Reanalysis 
ProteomicsAnalysis of mitochondrial N termini by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)
Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-28 | MSV000083504 | MassIVE
ProteomicsAn organellar Nα-acetyltransferase Naa60 acetylates cytosolic N-termini of transmembrane proteins and maintains Golgi integrity
N-terminal acetylation is a major and vital protein modification catalysed by N-terminal acetyltransferases (NATs). NatF or Nα-acetyltransferase 60 (Naa60) was recently identified as a NAT in higher eukaryotes. Here, we found that Naa60 differs from all other known NATs by its Golgi localization. A ...
ORGANISM(S): Homo sapiens (Human) 
2019-06-04 | PXD001155 | Pride
Naa60 N-terminal cofradic 
ProteomicsQuantitative N-terminome of N-end rule mutant in Arabidopsis
According to the /N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Here, we undertook a quantitative proteomics study of N-end rule mutant prt6, to investigate the impact of this pathway on the etiolated seedli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-10-27 | PXD006450 | Pride
N-terminome Arabidopsis N-end rule mutant etiolated seedlings tmt- labelling tails 
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana wt and vpe0 mutants
Comparison of protein termini in Arabidopsis thaliana vpe0 quadruple mutant and wildtype seedlings shortly after germination to identify differential processed proteins.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-03 | PXD012804 | Pride
Arabidopsis vpe0 termini degradomics hunter 
ProteomicsNAPT, an unbiased approach for sequential analysis of protein N- and C- terminome
Robust analysis of both protein N- and C-termini can reveal novel N- and C-degrons that modify protein stability and shape the eukaryotic proteome. Herein, we designed a workflow, termed LysargiNase-assisted Analysis of Protein N- and C-Terminome (NAPT) by sequential and selective separating N- and ...
ORGANISM(S): Homo Sapiens 
2022-04-11 | PXD030791 |
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