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ProteomicsHydrophobic tagging-assisted N-termini enrichment (HYTANE) for in-depth N-terminome analysis
The analysis of protein N-termini is of great importance for understanding the protein function and elucidating the proteolytic processing. Herein, we develop a negative enrichment strategy, termed as hydrophobic tagging-assisted N-termini enrichment (HYTANE) to achieve a global N-terminome analysis...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2016-08-19 | PXD004690 | Pride
Sacchoromyces cerevisiae Jurkat N-termini Caspase 
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana leaves using different proteases
Comparison of protein N-termini in Arabidopsis thaliana leaves by the HUNTER method; the corresponding digestion was performed with legumain, GluC and trypsin.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-20 | PXD014697 | Pride
Arabidopsis hunter n-termini profiling 
OtherAnnotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome.
Data from ProteomeXchange, PXD ID: PXD000434. Experiment: 111213_RBC5_MG, file: 111213_RBC5_MG_Fr15_17.mzml. Published as part of J Proteome Res. 2014 Apr 4;13(4):2028-44 . From the Abstract: {{i}} Enucleated and largely void of internal membranes and organelles, erythrocytes are simple yet proteom...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310000272 | GPMDB
Resource Reanalysis 
ProteomicsAnalysis of mitochondrial N termini by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)
Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-28 | MSV000083504 | MassIVE
ProteomicsN-terminome profiling in HEK293T
Various forms of the protein, called proteoform, are generated by co- or post-translational modification, alternative splicing and alternative translation initiation site, resulting in interactions between ribosomes, mRNAs, tRNAs, and various enzymes. Here, we introduce a N-terminal peptide enrichme...
ORGANISM(S): Homo sapiens (Human) 
2017-08-17 | PXD005583 | Pride
Protein stability Alternative translational initiation site Acetylation N-terminome Positional proteomics Proteoform N-termini Fasp N-end rule Translational initiation site 
ProteomicsAnalysis of sorted human peripheral blood mononuclear cells by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)
Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-28 | MSV000083503 | MassIVE
ProteomicsOptimization of HUNTER N-termini profiling using rat brain proteome extracts
Optimization hydrophobic tagging reaction time (30 min to 2h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified rat brain proteome as starting material.
ORGANISM(S): Rattus norvegicus (Rat) 
2019-09-03 | PXD012844 | Pride
Wistar rat hunter n-termini 
ProteomicsSensitive determination of proteolytic proteoforms in limited microscale proteome samples-Development of High-efficiency Undecanal-based N Termini EnRichment (HUNTER)
Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-28 | MSV000083501 | MassIVE
ProteomicsAnalysis of proteolytic processes in plasma and bone marrow interstitial fluid of pediatric B-ALL patients by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)
Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-28 | MSV000083502 | MassIVE
ProteomicsAn organellar Nα-acetyltransferase Naa60 acetylates cytosolic N-termini of transmembrane proteins and maintains Golgi integrity
N-terminal acetylation is a major and vital protein modification catalysed by N-terminal acetyltransferases (NATs). NatF or Nα-acetyltransferase 60 (Naa60) was recently identified as a NAT in higher eukaryotes. Here, we found that Naa60 differs from all other known NATs by its Golgi localization. A ...
ORGANISM(S): Homo sapiens (Human) 
2019-06-04 | PXD001155 | Pride
Naa60 N-terminal cofradic 
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