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ProteomicsCRISPR-assisted RNA-protein interaction detection (CARPID) combined with quantitative mass spectrometry was used to identify proteins interacting with the long non-coding RNA (lncRNA) Taurine Up-regulated 1 (TUG1) in HEK293T cells under physiological conditions and after Camptothecin (CPT) treatment.
This project utilized CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to identify proteins interacting with the long non-coding RNA (lncRNA) TUG1 in HEK293T cells. TUG1 preferentially interacts with known R-loop-associated proteins, particularly under Campt...
ORGANISM(S): Homo Sapiens (human) 
2025-07-17 | PXD049137 | JPOST Repository
ProteomicsProximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins
To characterize the protein interaction network of USP46, BioID analysis was conducted on a knock-in cell line that expresses USP46 and biotin ligase fusion protein stably.
ORGANISM(S): Mus Musculus (mouse) 
2025-03-25 | PXD053674 | JPOST Repository
ProteomicsAnalysis of thylakoid membrane proteome in Arabidopsis thaliana
To explore the role and target of chloroplast proteases under heat stress, thylakoid membranes were isolated from wild-type and mutant chloroplast thylakoid membrane-localized proteases after heat stress and subjected to comparative quantification by LC-MS/MS analysis using the spectral counting met...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2020-12-06 | PXD022899 | JPOST Repository
ProteomicsA novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain
To characterize the protein interaction network of USP46, BioID analysis was conducted on a knock-in cell line that expresses USP46 and biotin ligase fusion protein stably.
ORGANISM(S): Mus Musculus (mouse) 
2026-03-12 | PXD064961 | JPOST Repository
ProteomicsPosttranslational modification analysis of Histone H3t
Comprehensive posttranslational modifications in the testis-specific histone variant H3t protein validated in tagged knock-in mice.
ORGANISM(S): Mus Musculus (mouse) 
2024-09-13 | PXD052428 | JPOST Repository
ProteomicsCoRest’s acetylation acts as a temporal gate for activity-dependent transcription, limiting behavioral flexibility in Drosophila
In this project, to identify the proteins interacting with Rpd3 in the Drosophila mushroom body, the tandem affinity-purification(TAP) was performed. After in-solution digestion, the purified protein complex was analyzed by LC-MS/MS analysis using Orbitrap velos pro. PTM analysis was also performed...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2020-12-07 | PXD021294 | JPOST Repository
ProteomicsMouse Heart FLAG-Fam64a immunoprecipitation
Co-immunoprecipitation analysis using FLAG-Fam64a transgenic mouse heart.
ORGANISM(S): Mus Musculus (mouse) 
2022-04-28 | PXD020570 | JPOST Repository
ProteomicsCrosslinking MS of Elmo1,Dock5,Rac1 and RhoG complexes
Crosslink mass spectrometry of Elmo1,Dock5,Rac1 and RhoG complexes using BS3 crosslinker.
ORGANISM(S): Homo Sapiens (human) 
2025-01-18 | PXD048862 | JPOST Repository
ProteomicsIdentification of phosphorylation sites in human recombinant TOP2B
Human recombinant full-length TOP2B purified from HEK293 cells were phosphorylated with Erk1 and Erk2 in vitro. After in-gel digestion, the phosphorylation sites were determined using mass spectrometry.
ORGANISM(S): Homo Sapiens (human) 
2023-11-03 | PXD040977 | JPOST Repository
ProteomicsIdentification of the cross-linked ELMO1-DOCK5-Rac1 complex.
The ELMO1-DOCK5-Rac1 complex is a dimer of heterotrimers centered on a homodimer of the DOCK5 DHR-2 domain. We analyzed the ELMO1-DOCK5-Rac1 complex by cross-linking mass spectrometry and identified intermolecular cross-links between Rac1, ELMO1, and DOCK5.
ORGANISM(S): Homo Sapiens (human) 
2021-05-25 | PXD026097 | JPOST Repository
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