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Cocoa seed storage proteins play an important role in flavour development as aroma precursors are formed from their degradation during fermentation. Major proteins in the beans of Theobroma cacao are the storage proteins belonging to the vicilin and albumin fraction. Although both these classes of p...
ORGANISM(S): Theobroma cacao (Cacao) (Cocoa) 
2018-01-04 | PXD005586 | Pride
To investigate bioactivity of peptides identified in human plasma, we treated L6 myoblasts in vitro with a synthetic C-terminal peptide from TAGLN spanning amino acids 179-200, or a synthetic C-terminal peptide from TAGLN2 spanning amino acids 178-199 (1 µg/mL; 7 days), and compared the treatments t...
ORGANISM(S): Rattus norvegicus (Rat) 
2017-11-21 | PXD007176 | Pride
We further expanded on previous optimizations to isolate the plasma peptidome and compared various methods to maximize identifications with speed and ease. Previous studies have reported loss of polypeptides binding to high abundant proteins during depletion strategies. We hypothesized that rapid c...
ORGANISM(S): Homo sapiens (Human) 
2017-10-09 | PXD007191 | Pride
C57BL/6J mice were assigned to the hypobaric hypoxia chamber for simulation of high-altitude conditions. iTRAQ proteomics analysis/nanoUHPLC-MS/MS analysis was performed accordingly in the right myocardium of mice that had been allowed to develop hypoxia-induced pulmonary hypertension after hypobari...
ORGANISM(S): Mus Sp. 
2023-02-23 | PXD040342 |
HeLa cell line is frequently used in biomedical research, however little is known about N-glycan structures expressed on individual glycoproteins of this complex sample. We characterized site-specific N-glycosylation of HeLa N-glycoproteins using a complex workflow based on high and low energy tande...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2019-10-21 | PXD013930 | Pride
Voltage-Dependent Anion Channel isoforms (VDAC1, VDAC2, VDAC3) are relevant component of the outer mitochondrial membrane and play crucial role in cellular processes, in regulation of metabolism and in survival pathways. Recently, we reported the characterization of the oxidation pattern of cysteine...
ORGANISM(S): Rattus rattus (Black rat) 
2024-06-16 | PXD044041 | Pride
Four healthy male volunteers (age: 24 - 27; BMI: 24.2 - 25.9 kg/m2) underwent a single bout of high-intensity bicycle exercise reaching a VO2 max approximately 95% with blood samples and muscle biopsies taken immediately pre- and post-exercise. Subjects reached 95% VO2 max in approximately 9 to 12 m...
ORGANISM(S): Homo sapiens (Human) 
2016-06-29 | PXD001543 | Pride
Four healthy male volunteers (age: 24 - 27; BMI: 24.2 - 25.9 kg/m2) underwent a single bout of high-intensity bicycle exercise reaching a VO2 max approximately 95% with blood samples and muscle biopsies taken immediately pre- and post-exercise. Subjects reached 95% VO2 max in approximately 9 to 12 m...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080777 | MassIVE
Quantitative label-free proteome analysis of iPSC-derived immortalized megakaryocytes (imMKCLs), sorted by cell size and culture duration into six fractions, was performed in this project. Following tryptic digestion, peptide samples were analyzed by liquid chromatography–mass spectrometry (LC-MS). ...
ORGANISM(S): Homo Sapiens (human) 
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