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ProteomicsEndogenous pull-downs of human proteins ATP6V1A and AHNAK to validate interactions with influenza A virus proteins M2 and NEP by targeted mass spectrometry in virus-infected cells
Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry (AP-MS) and global phosphoprote...
ORGANISM(S): Homo sapiens (Human) 
2023-07-25 | PXD041663 | Pride
Virus-host Protein-protein interaction H5n1 Prm Human A549 Nhbe Influenza a virus 
GenomicsRNA-seq of differentiated primary normal human bronchoepithelial (NHBE) cells overexpressing ZNF676.
RNA-seq of differentiated primary normal human bronchoepithelial (NHBE) cells overexpressing ZNF676.
PRJNA686654 | ENA
GenomicsDiMNF treated NHBE cells with HCoV-OC43 Infection
DiMNF treated NHBE cells with HCoV-OC43 Infection
PRJNA994030 | ENA
ProteomicsStrain- and cell type-specific influenza A virus (IAV)-host protein-protein interaction (PPI) network identified by affinity purification-mass spectrometry (AP-MS)
Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and...
ORGANISM(S): Homo sapiens (Human) 
2023-07-25 | PXD036077 | Pride
Virus-host H5n1 Human Ppi H1n1 A549 Nhbe H3n2 Thp-1 Ap-ms Influenza a virus 
ProteomicsAnalysis of the apical secretome of Calu-3 and NHBE cells in 3D models of the human bronchial epithelium
The mucus secreted by airway epithelial cells plays a key role in the protection against and clearance of particles and pathogens. In this project, a 3D model of the bronchial epithelium was established using Calu-3 cells grown on porous inserts at the air-liquid interface. The secreted proteins wer...
ORGANISM(S): Homo sapiens (Human) 
2021-03-26 | PXD024242 | Pride
Human Lc-msms Calu-3 Nhbe Apical secretome Epithelial cells 
TranscriptomicsTranscription profiling of human normal human bronchial epithelial (NHBE) cells exposed to whole cigarette smoke over a time series to study the impact of tobacco exposure
Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke (CS) from a typical "full flavor" American brand of cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, which can ultimately define biomark...
ORGANISM(S): Homo sapiens 
2008-04-06 | E-GEOD-10718 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human NHBE cells exposed TO NORMAL human bronchial epithelial whole cigarette smoke FROM light AND standard brands
Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke from a reference cigarette (2R4F, University of Kentucky) and a typical American brand of "light" cigarettes ("Lights") in order to develop a better understanding of the genomic impact...
ORGANISM(S): Homo sapiens 
2008-04-06 | E-GEOD-10700 | biostudies-arrayexpress
TranscriptomicsEffect of cigarette smoke on normal human bronchial epithelial cells, and S9 toxicity
Temporal analysis of the effect of cigarette smoke on normal human bronchial epithelial cells (NHBE), and S9 toxicity.
ORGANISM(S): Homo sapiens 
2010-06-09 | E-GEOD-1276 | biostudies-arrayexpress
GenomicsPrimary normal human bronchial epithelial cells (NHBE) cultured at air liquid interface with or without macrophage cells (+/- Mac) derived from primary peripheral blood mononuclear cells
Primary normal human bronchial epithelial cells (NHBE) cultured at air liquid interface with or without macrophage cells (+/- Mac) derived from primary peripheral blood mononuclear cells
PRJNA660617 | ENA
TranscriptomicsLoss of cytoplasmic CDK1 predicts poor survival in human lung cancer and confers chemotherapeutic resistance
The dismal lethality of lung cancer is due to late stage at diagnosis and inherent therapeutic resistance. The incorporation of targeted therapies has modestly improved clinical outcomes, but the identification of new targets could further improve clinical outcomes by guiding stratification of poor-...
ORGANISM(S): Homo sapiens 
2011-03-31 | E-GEOD-28282 | biostudies-arrayexpress
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