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TranscriptomicsTranscription profiling of NIH3T3 cells over-expressing PPARg or EBF1 to study the efficiency and kinetics of cell adipogenesis induced by PPARg and EBF1.
NIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols. Keywords = Adipogene...
ORGANISM(S): Mus musculus 
2007-09-22 | E-GEOD-2192 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human NIH-3T3 US28-expressing and mock-transfected stablecell lines
The human cytomegalovirus (HCMV) encodes the chemokine receptor US28 that exhibits constitutive activity. NIH-3T3 cells stably transfected with US28 present a pro-angiogenic and transformed phenotype both in vitro and in vivo. We used gene expression profiling to determine how US28 constitutive acti...
ORGANISM(S): Mus musculus 
2009-04-04 | E-GEOD-13567 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse NIH3T3 Fibroblasts circadian expression, comparison with rhythmic gene expression in SCN2.2 Cells and the rat SCN
To screen for specific circadian outputs that may distinguish the pacemaker in the mammalian suprachiasmatic nucleus (SCN) from peripheral-type oscillators in which the canonical clockworks are similarly regulated in a circadian manner, the rhythmic behavior of the transcriptome in forskolin-stimula...
ORGANISM(S): Mus musculus 
2008-06-14 | E-GEOD-5810 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse NIH-3T3 cells treated with interferon alpha or gamma
Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1 or 3h on nt-RNA labeled for 30-60 min at different times of interferon treatment; Differential gene expression caused by IFN alpha or gamma was analyzed in newly transcribed RNA (nt-RNA) of NIH-3T3 cells...
ORGANISM(S): Mus musculus 
2009-10-11 | E-GEOD-9975 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human B-cells and mouse NIH-3T3 cells for analysis of RNA synthesis, abundance and decay
This SuperSeries is composed of the following subset Series:; GSE9973: Half-life determination for human B-cells (BL41); GSE9975: newly transcribed RNA (nt-RNA) for IFN alpha and gamma time course; GSE9977: Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma fo...
ORGANISM(S): Homo sapiens 
2009-10-10 | E-GEOD-10026 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse NIH-3T3 cells treated with mock, interferon alpha or gamma for 1or 3h
Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells compared to mock; We used microarrays to analyze the effects of 1 and 3h of IFNalpha and gamma treatment in total cellular RNA Experiment Overall Design: NIH-3T3 cells...
ORGANISM(S): Mus musculus 
2009-10-11 | E-GEOD-9977 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse NIH-3T3 cells for tc-, nt- and p-RNA half-life determination
Data from tc-, nt- and p-RNA as well as 1 and 2h of actinomycin-D treatment (5µg/ml) of NIH-3T3 cells used to determine half-lives. RNA was labeled for 15, 30 or 60 minutes with 4-thiouridine. After preparation of tc-RNA, thiol-labeled RNA was biotinylated using biot-HPDP and subsequently tc-RNA wa...
ORGANISM(S): Mus musculus 
2009-10-20 | E-GEOD-10011 | biostudies-arrayexpress
TranscriptomicsTranscriptional analysis of live and hybridized J1 and NIH-3T3 cells
J1 mouse embryonic stem cells (mESCs) and NIH-3T3 fibroblasts were grown in standard media conditions. Hybridized cells were fixed with 4% paraformaldehyde; incubated for 12 hours at 30C in an RNA preserving hybridization (RPH) buffer (300 mM Sodium chloride, 30mM Sodium citrate, 2.1M Ammonium sulfa...
ORGANISM(S): Mus musculus 
2014-03-20 | E-GEOD-55919 | biostudies-arrayexpress
TranscriptomicsExpression data from NIH-3T3 cells infected with MCMV for 2, 4 or 6h
Expression data from NIH-3T3 cells left uninfected or infected with MCMV for 2, 4 or 6h on total RNA as well as newly transcribed RNA labeled for 1-2, 3-4, and 5-6hpi. For newly transcribed RNA, the isolated RNA was labeled for 1h and separated from total cellular RNA following Trizol RNA preparatio...
ORGANISM(S): Mus musculus 
2013-02-01 | E-GEOD-35919 | biostudies-arrayexpress
ProteomicsMouse NIH-3T3 cohesin TurboID LC-MSMS
The cohesin complex is a plieotropic regulator of genome structure and function. It is known to play a role in sister chromatin cohesin, DNA replication, DNA repair, and regulation of gene expression. Cohesin has several known interacting proteins that aid in the various roles within a nucleus, yet ...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-02 | PXD060289 | Pride
Lc-msms Nih-3t3 Turboid Cohesin 
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