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ProteomicsMouse osteoclast secretory lysosomes nLC-ESI-MS/MS
To survey the proteome of osteoclast secretory lysosomes, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-25 | PXD037006 | Pride
Lysosome Lro Mouse Spion Osteoclast Secretory lysosome 
ProteomicsMouse osteoclast homogenates nLC-ESI-MS/MS - WT vs Slc37a2 KO
To survey the proteomic differences between WT and Slc37a2 knockout osteoclasts at the whole cell and secretory lysosome levels, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale mur...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-25 | PXD037910 | Pride
Lysosome Lro Mouse Spion Osteoclast Secretory lysosome 
ProteomicsMouse osteoclast secretory lysosomes nLC-ESI-MS/MS - WT vs Slc37a2 KO
To survey the proteome of osteoclast secretory lysosomes, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-25 | PXD037813 | Pride
Lysosome Lro Mouse Spion Osteoclast Secretory lysosome 
UnknownThe effects of frozen tissue storage conditions on the integrity of RNA and protein.
Not available
S-EPMC4213858 | biostudies-literature
UnknownEnrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants.
Not available
S-EPMC4560726 | biostudies-literature
GenomicsTranscriptome and proteome correlation during APETALA1-mediated activation of flower development in Arabidopsis
Transcriptome and proteome correlation during APETALA1-mediated activation of flower development in Arabidopsis
PRJNA899823 | ENA
ProteomicsProteomic characterization of brucellin: identifying key immunoreactive proteins for improved brucellosis diagnosis
This study used nLC-ESI-MS/MS analysis and bioinformatics tools to evaluate brucellin's protein composition and identify immunoreactive proteins.
ORGANISM(S): Brucella melitensis B115 
2026-02-09 | PXD063758 | Pride
Mass spectrometry Brucellosis Proteome Serological test Brucellin skin test 
ProteomicsIDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
Sumoylation is essential for progression through mitosis, but the specific protein targets and functions remain poorly understood. In this study, we used chromosome spreads to more precisely define the localization of SUMO-2/3 to the inner-centromere and protein scaffold of mitotic chromosomes. We a...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2014-10-07 | MSV000078890 | MassIVE
UnknownNano-LC FTICR tandem mass spectrometry for top-down proteomics: routine baseline unit mass resolution of whole cell lysate proteins up to 72 kDa.
Not available
S-EPMC3297430 | biostudies-literature
UnknownImproving the comprehensiveness and sensitivity of sheathless capillary electrophoresis-tandem mass spectrometry for proteomic analysis.
Not available
S-EPMC3498465 | biostudies-literature
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