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Single-cell RNA-seq (scRNA-seq) on nocodazole and DMSO treated cells before and after differentiation into endoderm. hPSC colonies were treated with DMSO or 100ng/ml nocodazole for 16 hours and induced to differentiate into definitive endoderm for three days. Single cells were subsequently collected...
ORGANISM(S): Homo sapiens 
Investigating the effect of microtubule disruption by nocodazole treatment on isolated adhesion complexes.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-19 | MSV000079926 | MassIVE
Human cells, either asynchronous or synchronized with nocodazole, were subjected to a pulldown assay using GST-maspin. Interacting proteins were eluted using 10 mM reduced glutathione, 150 mM NaCl, 1 mM DTT, and 50 mM Tris-HCl (pH 8.5).
ORGANISM(S): Homo sapiens (Human) 
2025-09-01 | PXD057847 | Pride
To explore the dynamics of integrin adhesion complex disassembly, isolated adhesion complexes from U2OS cells were subjected to mass spectrometry based proteomic analysis. U2OS cells were allowed to spread on FN, treated with nocodazole for 4 hours to disrupt microtubules. To coordinate microtubule-...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-20 | MSV000079967 | MassIVE
Analysis of synchronized HCT116 cells at various time points up to 10 hours following treatment with DMSO or Nocodazole. Experiment Overall Design: HCT116 colorectal cancer cell line was obtained from the American Type Culture Collection (ATCC) and grown in McCOY’S 5A MEDIUM MODIFIED (SIGMA) with ...
ORGANISM(S): Homo sapiens 
RNA BS-seq of Nocodazole treated HeLa cells with NSUN family member deficiency
Phosphoproteomic DIA-MS quantitative dataset for nocodazole treated cells (or “PhosDIA” in short). The MS samples injected in a previous publication for developing the IPF, an algorithm for identifying post-translationally modified peptides, were herein re-measured by a new, powerful Orbitrap Lumos ...
ORGANISM(S): Homo sapiens (Human) 
2020-06-08 | PXD017476 | Pride
ChIP-seq of H3K27Ac in the G1E-ER4 cell line in prometaphase (nocodazole block), between anaphase-telophase (nocodazole release 40min), and interphase (asynchronous). Nocodazole arrest-release in G1E ER4 cell line under conditions of 13h estradiol treatment.
ORGANISM(S): Mus musculus 
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