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ProteomicsFractionation of proteins on non-aqueous gradient
Tissue powder was separated on a non-aqueous gradient to fractionate proteins and metabolites for subcellular locations. In total, twelve fractions (01-12) were collected from three independent gradients (A, B, C)
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-24 | PXD000609 | Pride
Metabolite-protein interactions Subcellular location Non-aqueous fractionation 
MetabolomicsNon-targeted multimodal metabolomics and lipidomics data from ovine rumen fluid fractions

From an animal production and health perspective, our understanding of the metabolites in ruminant biofluids, particularly rumen fluid across different host species is poorly understood. Metabolomics is a powerful and sensitive approach for investigating low molecular weight metabolite profiles p...

2021-08-23 | MTBLS1717 | MetaboLights
UnknownNon-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development.
Not available
S-EPMC6804870 | biostudies-literature
ProteomicsProteomic analysis of aqueous humor and rejected graft in pig-to-nonhuman primate corneal xenotransplantation
Although pig-to-nonhuman primate (NHP) corneal xenotransplantation has shown long-term graft survival, xenogeneic antigenrelated immune responses are still stronger than allogenic antigen-associated responses. Therefore, there is an unmet need to investigate major rejection pathways in corneal xenot...
ORGANISM(S): Sus scrofa domesticus (domestic pig) Macaca mulatta (Rhesus macaque) 
2022-05-20 | PXD031320 | Pride
Aqueous humor Proteomics Cornea Nonhuman primate (macaque) Rejection Xenotransplantation Pig 
UnknownDissecting the subcellular compartmentation of proteins and metabolites in arabidopsis leaves using non-aqueous fractionation.
Not available
S-EPMC4159647 | biostudies-literature
ProteomicsArabidopsis thaliana non aqueous fractionation LC-MS/MS analysis of genotypes Ler (Landsberg erecta) and gin2-1 (glucose insensitive (hexokinase 1)) time series analysis under normal, chilling temperature and highlight+heat.
Plant cells are characterized by a high degree of compartmentalization and a diverse proteome and metabolome. Only a very limited number of studies has addressed combined subcellular proteomics and metabolomics which strongly limits biochemical and physiological interpretation of large-scale omics d...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-08-05 | PXD013646 | Pride
Arabidopsis thaliana Time series Subcellular protein marker Subcellular analysis Hexokinase 1 Cold Highlight Non aqueous fractionation Photorespiration Gin2-1 
UnknownNon-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues.
Not available
S-EPMC7853959 | biostudies-literature
UnknownSubcellular analysis of starch metabolism in developing barley seeds using a non-aqueous fractionation method.
Not available
S-EPMC3295393 | biostudies-literature
UnknownA Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome.
Not available
S-EPMC5177628 | biostudies-literature
ProteomicsNanodiamond Solid Phase Extraction and Triton X-114 Cloud Point Separation for Robust Fractionation and Shotgun Proteomics Analysis of Human Serum Proteome
Human serum is one of the most attractive specimens in biomarker research. However, its overcomplicated properties have hindered the analysis of low-abundance proteins by conventional mass spectrometry techniques. This work proposes an innovative strategy for utilizing nanodiamonds (NDs) in combinat...
ORGANISM(S): Homo sapiens (Human) 
2022-02-14 | PXD029710 | Pride
Proteomic analysis Cloud point separation Triton x-114 Prote-ome Nanodiamond Fractionation Solid phase extraction Human serum Lc-ms/ms 
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