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TranscriptomicsNASH
Non-alcoholic fatty liver disease (NAFLD) is characterized by a series of pathological changes that can progress from simple fatty liver disease to non-alcoholic steatohepatitis (NASH). The objective of this study is to describe changes in global gene expression associated with the progression of NA...
ORGANISM(S): Homo sapiens 
2011-07-12 | E-MEXP-3291 | biostudies-arrayexpress
TranscriptomicsMethylation profiling of different stages of human mammary epithelial cells from pre-statis to cancer
To characterize early epigenetic events in breast carcinogenesis, we analyzed DNA methylation state of different stages of HMECs from pre-stasis to cancer cell lines using human promoter microarray
ORGANISM(S): Homo sapiens 
2009-01-01 | E-MEXP-1889 | biostudies-arrayexpress
TranscriptomicsEMT
three pairs of isogenic human mammary epithelial and fibroblast cells, were used to characterized cell type specific dna methylation using using methyla cytosin immunoprecipitation
ORGANISM(S): Homo sapiens 
2013-01-17 | E-MEXP-3651 | biostudies-arrayexpress
ProteomicsHydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex
Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single ...
ORGANISM(S): Homo sapiens (Human) 
2021-12-30 | PXD021621 | Pride
Footprinting Hemoglobin Haptoglobin Fpop Timstof pro 
ProteomicsProteins of anti-sigma70 and anti-RNAP immunoprecipitates in bacteria
As part of the project aimed at the interaction of RNA with RNA polymerase and primary sigma factor in bacteria we analyzed anti-σ70 antibody immunoprecipitates from Mycobacterium smegmatis stationary phase to identify proteins in σA complex in non-treated cells. We performed mass spectrometry analy...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Corynebacterium glutamicum ATCC 13032 
2024-03-06 | PXD047705 | Pride
Sigma a Rnap Transcription Corynebacterium glutamicum Mycobacterium smegmatis 
TranscriptomicsMethylation profiling of normal and cancerous human breast samples
To assess changes in DNA methylation state associated with breast cancer we analyzed DNA from normal and cancerous breast specimens on CpG islands microarray using methylation-sensitive enzyme McrBC for target preparation. Cy3 was used for McrBC cut sample, Cy5 was used for reference Mock sample.
ORGANISM(S): Homo sapiens 
2006-11-03 | E-MEXP-881 | biostudies-arrayexpress
TranscriptomicsMethylation profiling of normal and cancerous breast cells from human patients and cell lines in a 125 kB region of the HOXA cluster
To evaluate DNA methylation profile associated with breast cancer in 125 kB region of HOXA cluster we analyzed DNA from normal cancerous breast specimens and cell lines. Analysis was performed on the tiling array covering entire HOXA region with 500bp resolution. Immunoprecipitation with anti-methyl...
ORGANISM(S): Homo sapiens 
2006-11-03 | E-MEXP-880 | biostudies-arrayexpress
ProteomicsPASEF technology on timsTOF Pro improves quality of FPOP analysis
Fast photochemical oxidation of proteins (FPOP) is a modern technique for studying protein folding, conformations, interactions, etc. In this work, timsTOF Pro mass spectrometer coupled with trapped ion mobility spectrometry was used for identification of oxidative modifications in a model protein. ...
ORGANISM(S): Homo sapiens (Human) 
2021-06-10 | PXD020509 | Pride
Tims Pasef Timstof Protein footprinting Hydroxyl radical Fpop 
ProteomicsRapid Detection of Procalcitonin in septic serum by Immunoaffinity MALDI Chips
PCT concentration in the bloodstream correlates well with sepsis presence and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-MS, that uses in-situ enrichment directly on ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD035456 | Pride
Immunoaffinity chips Procalcitonin Maldi-tof Sepsis 
TranscriptomicsNovel Post-radiation Sarcoma Cell Line
A primary human cell line, UACC-SARC1, was derived from a resection for a neoadjuvant chemotherapy resistant radiation induced spindle cell sarcoma. The sarcoma cell line was derived from a breast cancer patient who was treated with breast conserving surgery and radiation therapy. This radiation in...
ORGANISM(S): Homo sapiens 
2012-08-01 | E-MEXP-3419 | biostudies-arrayexpress
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