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Recent studies have revealed the physiological significance of post-translational lysine acylations in regulation of various cellular processes. Lysine succinylation is one of recently discovered post-translational acylations. To understand the distribution of lysine succinylations in five represen...
ORGANISM(S): Geobacillus kaustophilus HTA426 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (Rhodothermus obamensis) Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) Bacillus subtilis subsp. subtilis str. 168 
2016-12-14 | PXD002833 | Pride
To characterize the changes of phosphorylation signaling cascades and protein expressions induced by LAT1 inhibition in 4 cell lines of biliary tract cancer treated with LAT1 specific inhibitor JPH203, immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometry with ...
ORGANISM(S): Homo Sapiens (human) 
This study characterized the changes of phosphorylation signaling cascades induced by LAT1 inhibition in three cell lines of biliary tract cancer treated with LAT1 specific inhibitor JPH203 for 15 and 30 min. Immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometr...
ORGANISM(S): Homo Sapiens (human) 
Lauroylation-assisted N-terminomics for profiling protein N-termini, including ORF and non-ORF protein N-termini, using three biological replicates of etoposide-treated cholangiocarcinoma cells and exploiting negative selection.
ORGANISM(S): Homo Sapiens (human) 
(1) Lauroylation-assisted workflow for PICS using E. coli proteome-derived tryptic peptide libraries which were blocked (succinylation or dimethylation). (2) Lauroylation-assisted N-terminomics to profile protein N-termini, ORF and non-ORF protein N-termini, using etoposide-treated cholangiocarcinom...
ORGANISM(S): Homo Sapiens (human) Escherichia Coli 
The proteins expressed in normal human Schwann and sNF96.2 MPNST cells treated with EF1A1- and EF1A2-siRNA were profiled by SWATH-MS.
ORGANISM(S): Homo Sapiens (human) 
TCTP binding proteins were analyzed by SDS-PAGE gel-based protein identification.
ORGANISM(S): Homo Sapiens (human) 
TCTP binding proteins were identified by immunoprecipitation using anti-FLAG antibody and SWATH acquisition.
ORGANISM(S): Homo Sapiens (human) 
EF1A1- and EF1A2-binding proteins were identified by immuno-precipitation using anti-FLAG antibody and SWATH.
ORGANISM(S): Homo Sapiens (human) 
EF1A2-binding proteins in TCTP-deficient MPNST cells were identified by immuno-precipitation using anti-FLAG antibody and SWATH. To verify whether the change of the amount of EF1A2-binding proteins caused the cellular expression change, the proteins expressed in sNF96.2 MPNST cells treated with TCTP...
ORGANISM(S): Homo Sapiens (human) 
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