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UnknownSISCAPA peptide enrichment on magnetic beads using an in-line bead trap device.
Not available
S-EPMC2689780 | biostudies-literature
ProteomicsIdentification of ETV6 bound protein by affinity purification of ETV6-HA fusion protein in REH cells
To determine the protein partners of ETV6, we expressed ETV6 and HA-tagged ETV6 in Reh pre-B leukemic cells. Anti-HA magnetic beads were used for affinity purification of ETV6-HA (and ETV6 control) from the nuclear and cytoplasmic fraction. Following purification on-beads digest and LC-MS/MS experim...
ORGANISM(S): Homo sapiens (Human) 
2022-01-26 | PXD031001 | Pride
Leukemia All Etv6 
MetabolomicsVirus-encoded glycosyltransferases hypermodify DNA with diverse glycans

Enzymatic modification of DNA nucleobases can coordinate gene expression, nuclease protection or mutagenesis. We recently discovered a clade of phage-specific cytosine methyltransferase (MT) and 5-methylpyrimidine dioxygenase (5mYOX) enzymes that produce 5-hydroxymethylcytosine (5hmC) as a precur...

2024-08-22 | MTBLS10724 | MetaboLights
TranscriptomicsRNA-seq of human non-small-cell lung cancer cell line A549 treated with Feiyanning
We performed RNA seq to elucidate differential gene expression in A549 treated with Feiyanning (FYN). A549 cells were treated with FYN, then cultured for 24 h. Total RNA were extracted using TrizoI, and mRNA were retain using Oligotex mRNA Mini kit. The mRNA were fragmented into small fragments a...
ORGANISM(S): Homo sapiens 
2021-08-09 | E-MTAB-10821 | biostudies-arrayexpress
ProteomicsFLAG-PHF3 interactome in HEK293 cells
FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026292 | Pride
Human Anti-flag immunoprecipitation Nanolc-ms analysis On-bead digest 
ProteomicsPseudomonas putida SQ1 pull-down
Pseduomonas putida SQ1 cell lysates grown on sulfoquinovose (SQ) were labelled with activity-based probe 3 and labelled proteins pulled-down using streptavidin beads. An on-bead digest was performed and the resulting peptides analysed by LCMS/MS.
ORGANISM(S): Pseudomonas putida 
2024-07-10 | PXD047091 | Pride
Activity-based probe  sulfoquinovosidase  pseudomonas putida sq1 
MetabolomicsMicrobial liberation of N-methylserotonin from orange fiber in gnotobiotic mice and humans
Plant fibers in byproduct streams produced by non-harsh food processing methods represent biorepositories of diverse, naturally occurring, and physiologically active biomolecules. To demonstrate one approach for their characterization, mass spectrometry of intestinal contents from gnotobiotic mice, ...
2022-07-28 | MTBLS2331 | MetaboLights
ProteomicsRegional identity of human neural stem cells determinesoncogenic responses to histone 1H3.3 mutants
Regional identity of human neural stem cells determinesoncogenic responses to histone 1H3.3 mutants
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD021270 | Pride
Maxquant Q exactive plus Immunoprecipitation On-beads digest 
TranscriptomicsRNA-binding protein AUF1 promotes myogenesis by regulating MEF2C expression levels
The mammalian RNA-binding protein AUF1 (AU-binding factor 1, also known as heterogeneous nuclear ribonucleoprotein D, hnRNP D) binds to numerous mRNAs and influences their post-transcriptional fate. Given that many AUF1 target mRNAs encode muscle-specific factors, we investigated the function of AU...
ORGANISM(S): Mus musculus 
2014-12-22 | E-GEOD-58259 | biostudies-arrayexpress
ProteomicsArabidopsis SKD1 Proteom LC-MSMS
We investigated the heat-dependent in-vivo interactome of Arabidopsis thaliana SKD1 using a transgenic 35S::GFP-SKD1 line. Potential interactors of GFP-SKD1 were co-immunoprecipitated from cell extracts of untreated or heat-treated rosette leaves and analyzed by mass spectrometry. A line overexpress...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-06-23 | PXD025028 | Pride
Lc-msms Arabidopsis Heat stress Skd1 Proteom 
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