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ProteomicsC4PR_LIV Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer
Confident identification of sites of protein phosphorylation by mass spectrometry (MS) is essential to advance understanding of phosphorylation-mediated signaling events. However, development of novel instrumentation requires that methods for MS data acquisition and its interrogation be evaluated an...
ORGANISM(S): Homo sapiens (Human) 
2019-09-27 | PXD007058 | Pride
Mascot/ptmrs Andromeda/ptmscore Orbitrap fusion Mass spectrometry Phosphoproteomics 
ProteomicsBenchmarking multiple fragmentation methods on an Orbitrap Fusion for top-down phospho-proteoform characterization
Top-down analysis of intact proteins by mass spectrometry provides an ideal platform for comprehensive proteoform characterization, in particular, for the identification and localization of post-translational modifications (PTM) co-occurring on a protein. One of the main bottlenecks in top-down prot...
ORGANISM(S): Homo sapiens (Human) 
2015-07-16 | PXD001845 | Pride
Fragmentation Bora Ethcd Top down proteomics Phosphorylation Proteoform Orbitrap fusion 
ProteomicsPerformance of the Orbitrap Fusion Lumos Tribrid in single-shot analyses of human samples
One of the major additions in mass spectrometry technology has been the irruption of the Orbitrap mass analyzer, which has boosted the proteomics analyses of biological complex samples since its introduction. Here we assessed the performance of the new generation Orbitrap Fusion Lumos Tribrid mass s...
ORGANISM(S): Homo sapiens (Human) 
2017-03-09 | PXD004940 | Pride
Protein quantitation Orbitrap fusion lumos Mass spectrometry Phosphorylation Protein identification 
ProteomicsEvaluating cPILOT Performance on Orbitrap Instruments
A global cPILOT assay developed on an Orbitrap Velos instrument was transitioned to an Orbitrap Fusion Lumos instrument. Parameters such as the LC gradient, m/z isolation window, dynamic exclusion, targeted mass analyses, and SPS-N were optimized. The number of proteins identified on the Fusion Lumo...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD012133 | Pride
Proteomics Fusion lumos Alzheimer's disease Multiplexing Cpilot 
ProteomicsC4PR_LIV Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer
Confident identification of sites of protein phosphorylation by mass spectrometry (MS) is essential to advance understanding of phosphorylation-mediated signaling events. However, development of novel instrumentation requires that methods for MS data acquisition and its interrogation be evaluated an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-31 | MSV000085203 | MassIVE
ProteomicsOptimizing chromatographic conditions for orbitrap-based MS platforms
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
ORGANISM(S): Homo sapiens (Human) 
2019-03-04 | PXD009875 | Pride
Reproducibility Data-dependent acquisition Chromatography Q exactive Fusion lumos Human Pearson product-moment correlation coefficient Distributed normalized spectral abundance factor Orbitrap Quantitative proteomics Multidimensional protein identification technology 
ProteomicsHeLa, Orbitrap Fusion Lumos, Parameter optimization
Here, we report the optimization result of Orbitrap Fusion Lumos acquistion parameters.
ORGANISM(S): Homo Sapiens (human) 
2017-11-24 | PXD008282 | JPOST Repository
ProteomicsProtein Interactions of 7 MLL-fusion Proteins
MLL-fusions represent a large group of leukemia drivers, whose diversity originates from the vast molecular heterogeneity of C-terminal fusion partners of MLL protein. While studies of selected MLL-fusions have revealed critical molecular pathways, unifying mechanisms across all MLL-fusions remain p...
ORGANISM(S): Homo sapiens (Human) 
2018-05-25 | PXD009338 | Pride
Mll-fusion Leukemia Human 
ProteomicsCharacterization of sialylated N- and O-glycopeptides from complex proteomes on an Orbitrap Fusion Tribrid mass spectrometer by Isotope Targeted Glycoproteomics (IsoTaG). Application to azido and alkynyl sugars
Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation. Correlation of the intact glycoform to the protein attachment site is a critical step to assign functional roles to specific glycoproteins. Isotope targeted...
ORGANISM(S): Homo sapiens (Human) 
2017-02-22 | PXD004302 | Pride
Lc-ms/ms Tissue culture Glycoproteomics Metabolic labeling 
UnknownSystematic characterization of the components and molecular mechanisms of Jinshui Huanxian granules using UPLC-Orbitrap Fusion MS integrated with network pharmacology.
Not available
S-EPMC9304367 | biostudies-literature
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