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ProteomicsIdentification of TDP-43 - TAU interactions by XL-MS
Identification of TDP-43 - TAU interactions by XL-MS (DSS). The interactions were identified in for equimolar amount of TDP-43 - TAU and, as control, TDP-43 and MBP. After 30 minutes from the induction of phase separation, samples were either analyzed (bulk condition) or subjected to centrifugation ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD063858 | Pride
Xl-ms Tau Tdp-43 
ProteomicsFAIMS-GPF XL-MS: crosslinking-mass spectrometry based on gas-phase fractionation
Protein-protein interactions (PPIs) underpin nearly all cellular processes; therefore, mapping PPI and protein-protein association networks is critical for understanding how biological systems function in health and disease. However, many functionally relevant PPIs are transient and mediated by weak...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD064228 | Pride
Faims Cross-linking mass spectrometry Structural biology Interactomics 
UnknownImproving proteome coverage on a LTQ-Orbitrap using design of experiments.
Not available
S-EPMC3145359 | biostudies-literature
ProteomicsIdentification of ubiquitination site in BCL-XL after PROTAC DT2216 treatment
The availability of a suitable lysine for PROTAC-mediated ubiquitination can also determine the degradative ability of a protein target and the selectivity of a PROTAC. PROTAC DT2216 selectively triggers ubiquitination and degradation of BCL-XL. In this study, we identify K87 is the key ubiquitinate...
ORGANISM(S): Homo sapiens (Human) 
2019-10-08 | PXD015454 | Pride
Bcl-xl Ubiquitination site K87 Protac Dt2216 
UnknownIn Situ characterization of proteins using laserspray ionization on a high-performance MALDI-LTQ-Orbitrap mass spectrometer.
Not available
S-EPMC4224596 | biostudies-literature
ProteomicsDT2216 is a specific BCL-XL PROTAC
To validate the specificity of DT2216, we used the stable isotope labeling with amino acids in cell culture (SILAC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics to analyze the changes in proteins in WI-38 normal human diploid fibroblasts after DT2216 and DT2216NC tr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-08 | PXD010878 | Pride
Dt2216 bcl-xl protac silac lc-ms/ms 
ProteomicsPhosphorylation of Bcl-xL by ERK1
The anti-apoptotic function of Bcl-xL in the heart against reperfusion injury is diminished by K-Ras-Mst1-mediated phosphorylation of Ser14, which allows dissociation of Bcl-xL from Bax and promotes cardiomyocyte death. Here we show that Ser14 phosphorylation of Bcl-xL is also promoted by hemodynami...
ORGANISM(S): Homo sapiens (Human) 
2023-09-06 | PXD045118 | Pride
Human Phosphorylation Mitogen-activated protein kinase 3 Bcl-2-like protein 1 
ProteomicsDataset used to guide the development of Scout and bechmark XL-MS search engines
This submission includes the raw data analyzed and search results described in our manuscript “Proteome-Scale Recombinant Standards And A Robust High-Speed Search Engine To Advance Cross-Linking MS-Based Interactomics”. In this study, we develop a strategy to generate a well-controlled XL-MS standar...
ORGANISM(S): Homo sapiens (Human) 
2024-08-03 | PXD052022 | Pride
Search engine Cross-linking Ppi Benchmarking Xl-ms Fdr 
ProteomicsDevelopment and crystal structures of a more potent second-generation dual degrader of BCL-2 and BCL-xL
Overexpression of BCL-xL and BCL-2 play key roles in tumorigenesis and cancer drug resistance. Advances in PROTAC technology facilitated recent development of the first BCL-xL/BCL-2 dual degrader, 753b, a VHL-based degrader with improved potency and reduced toxicity compared to previous small molec...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-21 | MSV000094144 | MassIVE
ProteomicsBreaking Barriers in Crosslinking Mass Spectrometry: Enhanced Throughput and Sensitivity with the Orbitrap Astral Mass Analyzer
Crosslinking mass spectrometry (XL-MS) has become a key technique for investigating protein-protein interactions and the architecture of biological systems. This study compares the Orbitrap Astral and Eclipse mass spectrometers in XL-MS workflows, emphasizing their performance in detecting low-abund...
ORGANISM(S): Homo sapiens (Human) 
2025-12-15 | PXD059096 | Pride
Crosslinking mass spectrometry Fragmentation Orbitrap eclipse Hcd Quality control Compensation voltage Orbitrap astral 
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