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Human intervertebral disc tissue was obtained from patients (average age 51 yrs) undergoing surgery for lumbar interbody fusion (n=3) or lumbar disc herniation (n=1). Cells were isolated by sequential pronase-collagenase digestion [3]. Cells were passaged twice in monolayer and suspended at a densit...
ORGANISM(S): Homo sapiens 
In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through t...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhi 
Genomics
osmolarity induces hESC difference in several aspects
An SLC12A9-dependent ion transport mechanism regulates lysosomal osmolarity
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