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MetabolomicsParallelized multidimensional analytic framework, PAMAF, applied to mammalian cells uncovers novel regulatory principles in EMT
Not available
ORGANISM(S): Homo sapiens 
ST001861 | MetabolomicsWorkbench
ProteomicsA Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: Bulk HeLa Data Sets for PAMAF, pDIA-PAMAF, and DDA Modes
Here we present a novel data independent acquisition mass spectrometry operating mode termed parallel accumulation-mobility aligned fragmentation (PAMAF) that offers enhanced speed and sensitivity for ion fragmentation analysis for nontargeted discovery workflows such as bottom-up proteomics. This m...
ORGANISM(S): Homo sapiens (Human) 
2026-07-11 | PXD069730 | Pride
Human Pamaf Ion mobility Qtof Slim Hela Hrim Bottom-up proteomics 
ProteomicsA Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: HYE Quantification Data Sets for PAMAF Mode
Here we present a parallel accumulation-mobility aligned fragmentation (PAMAF) mode that expands the capabilities of the MOBILion Systems MOBIE® high-resolution ion mobility (HRIM) system for mass spectrometry-based proteomics. The MOBIE system leverages structures for lossless ion manipulation (SLI...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2026-07-11 | PXD078350 | Pride
Pamaf Ion mobility Qtof Slim Hye E. coli Yeast Hela Hrim Bottom-up proteomics 
ProteomicsA Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: Qual-Quan Data Sets for PAMAF and DDA Modes
Here we present a parallel accumulation-mobility aligned fragmentation (PAMAF) mode that expands the capabilities of the MOBILion® Systems MOBIE® high-resolution ion mobility (HRIM) system for mass spectrometry-based proteomics. The MOBIE system leverages structures for lossless ion manipulation (S...
ORGANISM(S): Homo sapiens (Human) 
2026-07-11 | PXD068773 | Pride
Human Pamaf Ion mobility Qtof Slim Hela Hrim Bottom-up proteomics 
ProteomicsA Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: Low-Load HeLa Data Sets for PAMAF and DDA Modes
Here we present a parallel accumulation-mobility aligned fragmentation (PAMAF) mode that expands the capabilities of the MOBILion Systems MOBIE® high-resolution ion mobility (HRIM) system for mass spectrometry-based proteomics. The MOBIE system leverages structures for lossless ion manipulation (SL...
ORGANISM(S): Homo sapiens (Human) 
2026-07-11 | PXD069951 | Pride
Human Pamaf Ion mobility Qtof Slim Hela Hrim Bottom-up proteomics 
ProteomicsParallel Accumulation with Mobility-Aligned Fragmentation (PAMAF) datasets for xTracer
This dataset contains raw mass spectrometry data acquired using Parallel Accumulation with Mobility-Aligned Fragmentation, PAMAF, a quadrupole-free DIA strategy with ultrahigh ion mobility resolution via SLIM. It includes two datasets, one with varying sample loads 25, 50, 100 ng, 45 min gradient an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-10-23 | MSV000099577 | MassIVE
GenomicsExpression data from MCF10A cells treated with TGFβ for multiple time points [miRNA-4]
We combine state-of-the-art data acquisition platforms and bioinformatics tools to devise PAMAF, a workflow that simultaneously examines twelve omics modalities, i.e., protein abundance from whole-cells, nucleus, exosomes, secretome and membrane; N-glycosylation, phosphorylation; metabolites; mRNA, ...
ORGANISM(S): synthetic construct Homo sapiens 
2022-11-11 | GSE194008 | GEO
TranscriptomicsExpression data from MCF10A cells treated with TGFβ for multiple time points [scRNA-seq]
We combine state-of-the-art data acquisition platforms and bioinformatics tools to devise PAMAF, a workflow that simultaneously examines twelve omics modalities, i.e., protein abundance from whole-cells, nucleus, exosomes, secretome and membrane; N-glycosylation, phosphorylation; metabolites; mRNA, ...
ORGANISM(S): Homo sapiens 
2022-11-11 | GSE200981 | GEO
TranscriptomicsExpression data from MCF10A cells treated with TGFβ for multiple time points [HuGene-2_0]
We combine state-of-the-art data acquisition platforms and bioinformatics tools to devise PAMAF, a workflow that simultaneously examines twelve omics modalities, i.e., protein abundance from whole-cells, nucleus, exosomes, secretome and membrane; N-glycosylation, phosphorylation; metabolites; mRNA, ...
ORGANISM(S): Homo sapiens 
2022-11-11 | GSE193985 | GEO
UnknownParallelized multidimensional analytic framework applied to mammary epithelial cells uncovers regulatory principles in EMT.
Not available
S-EPMC9908882 | biostudies-literature
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