Passaging - OmicsDI

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Instrument Platform

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JANUS® G3 MDT automated liquid handling workstation (PerkinElmer); thermal cycler or incubator (42 °C); Zymo DNA Clean & Concentrator columns; AMPure XP magnetic bead purification system; magnetic separation rack. (1)
Multichannel pipettes or automated liquid handler; 384-well tissue culture plates; Class II biological safety cabinet; CO₂ incubator (37 °C, 5% CO₂). (1)
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Publication Date

2014 (36)
2015 (35)
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2008 (8)
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2006 (3)
2007 (2)

CHEBI ID

CHEBI:17992 (2)
CHEBI:28123 (2)
CHEBI:189567 (1)
CHEBI:189566 (1)
CHEBI:189565 (1)
CHEBI:189564 (1)
CHEBI:157853 (1)
CHEBI:151217 (1)
CHEBI:157801 (1)
CHEBI:59563 (1)
CHEBI:189571 (1)
CHEBI:180917 (1)
CHEBI:90330 (1)
CHEBI:141457 (1)
CHEBI:28134 (1)
CHEBI:157862 (1)
CHEBI:73762 (1)
CHEBI:141439 (1)
CHEBI:73804 (1)
CHEBI:16710 (1)
CHEBI:189568 (1)
CHEBI:74052 (1)
CHEBI:134468 (1)
CHEBI:141455 (1)
CHEBI:90354 (1)
CHEBI:73801 (1)
CHEBI:166843 (1)
CHEBI:63357 (1)
CHEBI:74808 (1)
CHEBI:75009 (1)

Metabolite Name

Betaine (2)
Diethanolamine (2)
2-(6-hydroxy-6-methyloctyl)-2H-furan-5-one (1)
[3-[2-aminoethoxy(hydroxy)phosphoryl]oxy-2-[(4Z,7Z)-octadeca-4,7-dienoyl]oxypropyl] octadecanoate (1)
PS(O-18:0/12:0) (1)
PS(P-18:0/12:0) (1)
2-(1-Methylpropyl)-4,6-dinitrophenol (1)
DL-alpha-Lipoic Acid (1)
DL-3-hydroxy-3-methylglutaryl-CoA (HMG CoA) (1)
Dihydroxyfumarate (1)
D-Glucosamine 1-phosphate (Glucosamine-1P) (1)
Deoxycytidine 5'-triphosphate (dCTP) (1)
2'-Deoxycytidine diphosphate (dCDP) (1)
P1,P4-Diadenosine 5'-tetraphosphate (1)
Malvidin 3-O-glucoside (cation) (1)
GDP-L-Fucose (1)
.alpha.-D-(+)-Talose (1)
4-O-beta-Galactopyranosyl-D-mannopyranose (1)
2-Deoxy-D-glucose 6-phosphate (1)
Allantoate/Allantoic acid (1)
PI-Cer(t18:0/20:0(2OH)) (1)
Pyridoxine 5-phosphate (1)
Ile-Cys (1)
1-Stearoyl-rac-glycerol (1)
3-Guanidinopropanoate (1)
Cys-Ala (1)
Tetrahydro-L-biopterin (1)
Thr-Gln (1)
DL-2,4-Diaminobutyric acid (1)
Uridine diphosphate glucose (UDP-D-Glucose) (1)

First Public Date

2025 (59)
2024 (53)
2026 (47)
2023 (37)
2014 (37)
2021 (34)
2022 (29)
2020 (19)
2018 (19)
2015 (16)
2019 (13)
2017 (10)
2013 (9)
2016 (7)
2011 (2)

Classification

viruses (18)
bacteria (15)
fungi (3)

Study type

Transcription profiling by array (102)
RNA-seq of coding RNA (53)
RNA-seq of coding RNA from single cells (19)
ChIP-seq (14)
Comparative genomic hybridization by array (11)
Methylation profiling by array (9)
Cell line - High-throughput sequencing (7)
DNA-seq (7)
Unknown experiment type (5)
Other (4)
Methylation profiling by high throughput sequencing (4)
RNA-seq of total RNA (3)
Genotyping by array (2)
Baseline (1)
Differential (1)
Human - Two-color microarray (1)
Human - Methylation microarray (1)
ChIP-chip by tiling array (1)
Human - High-throughput sequencing (1)
Plant - High-throughput sequencing (1)
Cell line - One-color microarray (1)
Trajectory (1)
ChIP-chip by array (1)
Transcription profiling by RT-PCR (1)
MicroRNA profiling by high throughput sequencing (1)
Spatial transcriptomics by high-throughput sequencing (1)
ScATAC-seq (1)
MicroRNA profiling by array (1)
Genotyping by high throughput sequencing (1)
RNA-seq of non coding RNA (1)

Release Date

2022 (119)
2025 (98)
2021 (98)
2024 (90)
2020 (88)
2023 (84)
2019 (71)
2018 (65)
2016 (62)
2015 (62)
2014 (62)
2013 (58)
2017 (55)
2011 (39)
2012 (33)
2010 (31)
2009 (21)
2026 (19)
2008 (15)
2005 (10)
2000 (5)
2002 (4)
2007 (3)
2006 (3)
2004 (2)
1998 (2)
2003 (1)
2001 (1)
1997 (1)

Lab affiliation

Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea (2)
General Manager of PROMEC Department of Clinical and Molecular Medicine +4773598508 +4772573342 Laboratoriesenteret, 231.04.008, Øya, Erling Skjalgssons gate 1 Norway (2)
2Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, Madison, WI 53705, USA (1)
Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA (1)
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center (1)
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA (1)
Department of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, China (1)
Department of Molecular and Applied Microbiology Leibniz Institute for Natural Product Research and Infection Biology - Hans KnÃ¶ll Institute Jena Leibniz-HKI Adolf-Reichwein-Str. 23 Jena, Germany (1)
Department of Pediatrics, Clinical Sciences Lund, Lund University, Lund, Sweden (1)
Department of Pharmaceutical Sciences, University of Illinois at Chicago (1)
Experimental Radiation Oncology department, The University of Texas MD Anderson Cancer Center (1)
Gregor Mendel Institute of Molecular Plant Biology GmbH Dr. Bohr-Gasse 3, 1030 Vienna, Austria Phone: +43 1 79044 9850 http://www.gmi.oeaw.ac.at (1)
Institute of Microbiology, The Czech Academy of Sciences (1)
Laboratory of Regenerative Biomedicine, Institute of Cytology of the RAS (1)
Medical Mycology Laboratory, Department of Biology, Maynooth University (1)
Microbiology department at the Icahn School of Medicine atMount Sinai (1)
Military Health Institute, Prague, Czech Republic (1)
Nuffield department of Surgical Sciences, University of Oxford UK (1)
Ocean University of China (1)
Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Associate Editor, ACS Chemical Biology (1)
Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Exceutive Editor, ACS Chemical Biology (lab head) (1)
Professor in molecular biology and scientific leader of PROMEC Department of Clinical and Molecular Medicine Faculty of Medicine and Health Sciences +4773598608 +4791825455 +4772573076 Laboratoriesenteret, 231.05.034, Øya, Erling Skjalgssons g 1 Trondheim 7030, Norway (1)
The Fifth Affiliated Hospital of Guangzhou Medical University (1)
Wellcome Trust - MRC Cambridge Stem Cell Institute Anne McLaren Laboratory and Department of Surgery, University of Cambridge, UK. Wellcome Trust Sanger Institute, Hinxton UK. (1)
ZMBH University Heidelberg Im Neuenheimer Feld 282 69120 Heidelberg Germany (1)
ZMBH, University of Heidelberg Im Neuenheimer Feld 282 69120 Heidelberg (1)

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A stress-reduced passaging technique improves the viability of human pluripotent cells

Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more effic...

ORGANISM(S): Homo Sapiens (human)

2022-01-23 | PXD028731 | JPOST Repository

Expression of four hESC lines in enzymatic and mechanic passaging methods

hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition. Here transcriptome of four different hESC lines were compared in two passaging methods.

ORGANISM(S): Homo sapiens

2015-06-01 | GSE47916 | GEO

Genomic instability of human embryonic stem cells in mechanic and enzymatic passaging culture

hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition. Here sub-karyotypic genome change in three different hESC lines were compared in two passaging methods.

ORGANISM(S): Homo sapiens

2015-06-01 | GSE47914 | GEO

Transcription profiling of pig coronary arterial endothelial cells by multiple passaging in vitro

Nitric oxide helps to prevent endothelial dysfunction and senescence. This study aimed to define genomic and proteomic changes in cultured porcine senescent endothelial cells and their resemblance with those observed in regenerated endothelial cells. Senescent endothelial cells were produced by pass...

ORGANISM(S): Sus scrofa

2008-06-16 | E-GEOD-8974 | biostudies-arrayexpress

Serial passaging of Aspergillus fumigatus on Galleria extract agar produces strains with altered response of oxidative stress

Aspergillus fumigatus is an environmental saprophyte and opportunistic fungal pathogen of the human lung. Serially passaging of A. fumigatus on an agar produced from Galleria mellonella larvae produced strains showing alterations in virulence, anti-fungal susceptibility and in protein abundances tha...

ORGANISM(S): Aspergillus fumigatus Af293

2023-05-10 | PXD036787 | Pride