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ProteomicsArabidopsis thaliana peptide SP2 and ethyl acetate extraction clean up.
The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, the single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-03 | PXD045031 | Pride
Arabidopsis thaliana Peptide clean-up Ethyl acetate extraction Sp2 Polyethylene glycol Sodium dodecyl sulfate Magnetic beads Lc-ms/ms 
TranscriptomicsInhibition of Bcl6-SMRT/NCoR interactions by an inhibitory peptide affects inflammatory pathways
Using microarrays, we compared the changes in levels of gene expression between wild type mouse bone marrow derived macrophages upon treatment with the Bcl6 peptide inhibitor, RI-BPI, that specifically blocks interaction between BCL6 and the co-repressors NCoR or SMRT. Total RNA was obtained from cu...
ORGANISM(S): Mus musculus 
2012-03-29 | E-GEOD-27001 | biostudies-arrayexpress
UnknownImmunocapture sample clean-up in determination of low abundant protein biomarkers - a feasibility study of peptide capture by anti-protein antibodies.
Not available
S-EPMC9097496 | biostudies-literature
UnknownBenchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS.
Not available
S-EPMC10743447 | biostudies-literature
Proteomics3D-Printed Device for Consistent and High-Throughput Peptide Sample Clean-up Using StageTips
Mass spectrometry (MS) is a fundamental technology for the study of proteomics. A core technique for MS analysis is solid-phase extraction (SPE) for the removal of contaminants and sample concentration, and StageTips are one of the most commonly used devices used for SPE in modern proteomics workflo...
ORGANISM(S): Homo sapiens (Human) 
2019-03-21 | PXD009324 | Pride
3d-printed Stagetip 96-well 
TranscriptomicsTranscription profiling of human CD14+ monocytes treated with innate defence regulator peptide IDR-1 in the presence/absence of lipopolyssacharide
Effect of peptide IDR-1 on CD14+ human monocytes. >br< >br< Effect of peptide IDR-1 on LPS-induced responses in CD14+ human monocytes.>br< >br< Human peripheral blood mononuclear cells (PBMC) were treated with innate defence regulator peptide IDR-1 in the presence and absence of LPS (purified from P...
ORGANISM(S): Homo sapiens 
2007-04-10 | E-FPMI-8 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of endotoxin-stimulated human monocytic cells in presence and absence of host defense peptide LL-37
The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence and absence of host defense peptide LL-37. A functional genomics approach was used to establish a temporal transcriptional profile and identify differentially expressed gene...
ORGANISM(S): Homo sapiens 
2006-08-01 | E-FPMI-4 | biostudies-arrayexpress
ProteomicsSPE optimization for peptide sample preparation for urine peptidomics
Development of a modified solid phase extraction (SPE) approach for improved peptide yield and analysis sensitivity with LC-MS based peptidomics of urine.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-23 | MSV000080086 | MassIVE
ProteomicsReduction of Interference Ions Generated in the Enrichment Process of Biotinylated Peptides using Tamavidin 2-REV
After tryptic digestion of proteins of RAW264.7 cells expressing TurboID-STING, biotinylated peptides were captured with Tamavidin 2-REV magnetic beads and eluted competitively with excess biotin. When the peptide samples were subjected to LC-MS/MS, high intensity peaks of interference and/or contam...
ORGANISM(S): Cellular Organisms Mus Musculus (mouse) 
2022-08-25 | PXD035218 | JPOST Repository
TranscriptomicsTranscription profiling of human monocytic cells THP-1 stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours
The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence or absence of host defense peptide LL-37 at low physiologically relevant concentrations. Human monocytic cells THP-1 were stimulated with LPS (10ng/ml) in presence or absenc...
ORGANISM(S): Homo sapiens 
2006-12-07 | E-FPMI-6 | biostudies-arrayexpress
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