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MetabolomicsSerine synthesis pathway enzyme PHGDH is critical for muscle cell biomass, anabolic metabolism and mTORC1 signaling

Cells use glycolytic intermediates for anabolism e.g., via the serine synthesis and pentose phosphate pathways. However, we still understand poorly how these metabolic pathways contribute to skeletal muscle cell biomass generation. The first aim of this study was therefore to identify enzymes tha...

2025-03-21 | MTBLS8854 | MetaboLights
TranscriptomicsTranscription profiling of murine embryonic head Phgdh knock outs
D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) is a necessary enzyme for de novo L-serine biosynthesis via the phosphorylated pathway. We demonstrated previously that Phgdh is expressed exclusively by neuroepithelium and radial glia in developing mouse brain and later mainly by astrocytes. ...
ORGANISM(S): Mus musculus 
2008-06-17 | E-GEOD-8555 | biostudies-arrayexpress
ProteomicsDetermine G6PD/PHGDH deamidation in HepG2 cells with CAD depletion or in cell cycles
We report here that the trifunctional carbamoyl-phosphate synthase, aspartate transcarbamoylase, and dihydroorotase (CAD) activates the pentose phosphate pathway (PPP) and serine synthesis pathway (SSP) to promote de novo nucleotide synthesis via deamidation. Biochemical analyses show that CAD inter...
ORGANISM(S): Homo sapiens (Human) 
2026-02-03 | PXD055217 | Pride
Liver G6pd Phgdh Cad Deamidation 
ProteomicsDeamidation sites of G6PD and PHGDH catalyzed by CAD
CAD interacts with and deamidates glucose-6-phosphate dehydrogenase (G6PD) and phosphoglycerate dehydrogenase (PHGDH), which are rate-limiting enzymes of the PPP and SSP, respectively. Here we identified G6PD and PHGDH deamidation sites by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2026-02-03 | PXD052165 | Pride
G6pd Phgdh Cad Deamidation 
ProteomicsThe proteins that PHGDH interact with in mitchondria
To determine the proteins that interact with PHGDH in mitchondria, we overexpressed Flag-PHGDH in cells and immunoprecipitation of PHGDH with Flag antibody. Mass spectrometry analysis of the PHGDH immunoprecipitation mixture and determined the mitochondrial proteins that interact with PHGDH.
ORGANISM(S): Homo sapiens (Human) 
2022-10-06 | PXD036321 | Pride
Phgdh Mitochondrial proteins 
ProteomicsIdentification of lactoylation on PHGDH
Sites of lactoylation were identified on both recominant human PHGDH and PHGDH in cellulo. For recombinant protein, sample was treated with 100 uM LGSH for 24 hours. For in cellulo, cells were transfected with PHGDH-mycDDK and treated with 50 uM MGO for lactoylLys identification.
ORGANISM(S): Homo sapiens (Human) 
2026-03-09 | PXD060533 | Pride
Phgdh Serine 
ProteomicsBiophysical and biochemical properties of PHGDH revealed by studies on PHGDH inhibitors
Biophysical and biochemical properties of PHGDH revealed by studies on PHGDH inhibitors
ORGANISM(S): Homo Sapiens 
PXD029445 |
ProteomicsIP-MS detected the interation proteins of PHGDH
Vascular endothelial cells (ECs) senescence correlates with the increase of cardiovascular diseases in ageing population. Although ECs rely glycolysis for energy production, little is known about the role of glycolysis in ECs senescence. Here, we report a critical role for glycolysis-derived serine ...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD035586 | Pride
Mass spectrometry Phgdh Co-immunoprecipitation 
ProteomicsNon-metabolic PHGDH facilitates cMyc to urge advanced liver cancer
Metabolic dysregulation is an important feature of malignant tumors. Serine synthesis pathway (SSP) reshapes tumor cells to enhance their growth and survival capabilities, especially under serine starvation. However, whether and how SSP is involved in the progression of liver cancer has not been cle...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-20 | PXD017809 | Pride
Serine synthesis Hepatocellular carcinoma Phgdh Proteome 
GenomicsPHGDH heterogeneity potentiates cancer cell dissemination and metastasis
PHGDH heterogeneity potentiates cancer cell dissemination and metastasis
PRJNA814732 | ENA
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