Sort   by:  
 Page size 
ProteomicsZirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple an...
ORGANISM(S): Homo sapiens (Human) 
2020-11-26 | PXD018273 | Pride
Affinity enrichment Moac Imac Mass spectrometry Protein phosphorylation Functional proteomics Cell signalling Magnetic particles 
Proteomics,MultiomicsProtein phosphorylation evolution across 18 fungal species
Protein phosphorylation evolution across 18 fungal species. For each species whole cell extracts were digested separately with trypsin and LysC. Each digest was IMAC enriched and analyzed by LC-MS/MS in technical triplicate on an LTQ Orbitrap Velos using a 120min method. Data was searched against th...
ORGANISM(S): S. Cerevisiae, S. Paradoxus, S. Mikatae, S. Kudriavzevii, S. Bayanus, N. Castellii, C. Glabrata, V. Polyspora, Z. Rouxii, K. Lactis, L. Kluyveri, L. Thermotolerans, L. Waltii, K. Pastoris, M. Guilliermondii, C. Albicans, S. Stipitis, S. Pombe 
2015-12-18 | MSV000079428 | MassIVE
OtherReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC.
Data from ProteomeXchange, PXD ID: PXD000892. File: FIG5_1_KRAS_G12D_3.msf.mgf. Published as part of Anal Chem. 2014 Sep 18 . From the Abstract: {{i}} Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Here, we describe the application o...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320003560 | GPMDB
Resource Reanalysis 
ProteomicsTIHAMAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Analysis
Protein post-translational modifications (PTMs) are pivotal molecular events in plant cells, encompassing processes such as protein folding and enzyme activation. Among these PTMs, N-glycosylation and phosphorylation hold particular importance, as they play essential roles in regulating plant growth...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-04-03 | PXD047065 | Pride
N-glycoproteomics Hilic Arabidopsis thaliana Imac Aba signaling Phosphoproteomics 
ProteomicsDefeating major contaminants in Fe3+-IMAC phosphopeptide enrichment
Optimized sample preparation workflow for Fe-IMAC phosphopeptide enrichment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-02-19 | PXD008289 | Pride
Human E.coli 
ProteomicsIntegrated Single-Tip IMAC-HILIC Enrichment Enables Simultaneous and Efficient Analysis of Plant Phosphoproteomics and N-glycoproteomics
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
Phosphoproteomics  n-glycoproteomics  enrichment  immobilized metal affinity chromatography  hydrophilic interaction chromatography 
ProteomicsAnalysis of protein phosphorylation in response to the ATP treatment in Arabidopsis thaliana
The project intended to reveal protein phosphorylation patterns in Arabidopsis thaliana in response to ATP. For this purpose, Arabidopsis thaliana plants, including WT, ATP receptor mutants (p2k1, p2k2, and double mutant p2k1/p2k2), and P2K1 overexpression plants, were treated with ATP or buffer (as...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000095888 | MassIVE
ProteomicsNELF-A phosphorylation site identification
NELF-A phosphorylation by P-TEFb is a key event in the pausing release. We conduct IMAC-LC-MS-MS to identified NELF-A phosphorylation site by activation P-TEFb kinase.
ORGANISM(S): Homo sapiens (Human) 
2016-03-23 | PXD003433 | Pride
Imac Nelf-a Hela cell Human 
ProteomicsProtein phosphorylation landscape of mouse spermatids during spermiogenesis
Tadpole-shaped sperm is formed from round spermatid by spermiogenesis, a process with dramatic morphological changes in the final stage of spermatogenesis in testis. Protein phosphorylation, as one of the most important post-translational modifications, can regulate spermiogenesis, however, there is...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-29 | PXD011890 | Pride
Mouse spermatids Spermiogenesis Phosphorylation 
ProteomicsTi4+-IMAC label-free quantification
We combine high-resolution mass spectrometry with Ti4+-IMAC phosphopeptide enrichment and label-free quantification to monitor the phosphoproteome of Jurkat T-cells following stimulation by Prostaglandin E2. Jurkat T lymphoma cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine se...
ORGANISM(S): Homo sapiens (Human) 
2014-05-22 | PXD000293 | Pride
Label-free phosphoproteomics 
Sort   by:  
 Page size