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ProteomicsC9ORF72 repeat expansion affects the proteome of primary skin fibroblasts in ALS and FTD
The study design involves the proteomics characterization of primary skin fibroblast cell lines derived from skin biopsies from patients affected by either amyotrophic lateral sclerosis (ALS) or frontotemporal dementia (FTD). Patients enrolled were divided in three groups: 1) ALS patients carrying t...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD023866 | Pride
Human Hdmse Als Fibroblast 
ProteomicsAge-Linked Variations in Adipose Tissue Responses to Nerve Injury in a murine model: Sex-Specific Patterns Over Time
Adipose tissue (AT) plays a central role in the metabolic and inflammatory responses to neuropathy. It secretes adipokines, such as leptin and adiponectin, which regulate nerve regeneration and inflammation . Postmenopausal women have a higher prevalence of PN, linked to estrogen depletion, which in...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-10 | PXD061152 | Pride
Gender Mouse Lc-ms 
ProteomicsExploring the HeLa Dark Mitochondrial proteome
In the framework of the Human Proteome Project initiative, we aim to improve mapping and characterization of mitochondrial proteome. In this work we implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochon...
ORGANISM(S): Homo sapiens (Human) 
2020-02-27 | PXD014201 | Pride
Personalised medicine Human mitochondria Dark proteins 
ProteomicsExploring the HeLa Dark Mitochondrial proteome
In the framework of the Human Proteome Project initiative, we aim to improve mapping and characterization of mitochondrial proteome. In this work we implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochon...
ORGANISM(S): Homo sapiens (Human) 
2020-02-27 | PXD014200 | Pride
Personalised medicine Subproteome Mass spectrometry Mitochondria Drug targets Dark proteome 
ProteomicsMitochondrial proteomics investigation of a cellular model of impaired dopamine homeostasis, an early step in Parkinson's disease pathogenesis
Impaired dopamine homeostasis is an early event in the pathogenesis of Parkinson's disease. Generation of intracellular reactive oxygen species consequent to dopamine oxidation leads to mitochondrial dysfunction and eventually cell death. Alterations in the mitochondrial proteome due to dopamine exp...
ORGANISM(S): Homo sapiens (Human) 
2014-04-15 | PXD000838 | Pride
Dopamine  human proteome project  mitochondria  mpp+  parkinson's disease  respiratory chain  shotgun proteomics  mse 
ProteomicsExploring the impact of PARK2 mutations on the total and mitochondrial proteome of human skin fibroblasts
Mutations in PARK2 gene are the most frequent cause of familial forms of Parkinson’s disease (PD). This gene encodes Parkin, an E3 ubiquitin ligase involved in several cellular mechanisms, such as the mitophagic process. Mutations in this gene, which cause the loss of function of Parkin, are respons...
ORGANISM(S): Homo sapiens (Human) 
2020-05-11 | PXD015880 | Pride
Parkinson’s disease Shotgun proteomics Mass spectrometry Human skin fibroblast Park2 mutation Mitochondria Immunoprecipitation 
ProteomicsSequential Fractionation Strategy Identifies Nine Missing Proteins in The Mitochondrial Proteome of Commonly Used Cell Lines
Identification of mitochondrial proteins with a bottom up approach after organelle enrichment and 1D PAGE separation for 5 different cell lines. The bands were cut and the proteins reduced, alkylated and digested with trypsin according to a conventional protocol. Acquisition were performed on the Or...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-05-28 | MSV000082409 | MassIVE
ProteomicsStandardization of mitochondrial proteomics v2 NP
Comparison of the extraction performances of three different mitochondria enrichment protocols (differential centrifugation, sucrose gradient, a commercial kit based on surfactants) on ten different cell lines. Samples were analysed on several instrumental platforms. Mitochondrial pellets were lysed...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-07-14 | MSV000081329 | MassIVE
ProteomicsStandardization of mitochondrial proteomics
Comparison of the extraction performances of three different mitochondria enrichment protocols (differential centrifugation, sucrose gradient, a commercial kit based on surfactants) on ten different cell lines. Samples were analysed on several instrumental platforms. Mitochondrial pellets were lysed...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-05-30 | MSV000081121 | MassIVE
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