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MetabolomicsSeminal lipid depletion and gut microbiome–metabolome alterations in men with gonadotropin-treated idiopathic hypogonadotropic hypogonadism: an integrated multi-omics study
This study investigated seminal plasma lipid alterations and gut microbiome–metabolome changes in men with gonadotropin-treated idiopathic hypogonadotropic hypogonadism. Seminal plasma metabolomics, fecal metagenomic sequencing, and fecal metabolomics were performed in patients and healthy controls....
2026-07-20 | MTBLS15080 | MetaboLights
ProteomicsiEvaluation of spin columns for human plasma depletion to facilitate MS-based proteomics analysis of SARS-cov-2 positive plasman columns for human plasma depletion to facilitate MS-based proteomics analysis of SARS-cov-2 positive plasma
the benefit on the number of identified proteins is well documented and the depletion step remains widely used in in-depth proteomics. Recently, single-use depletion spin columns have been developed (i.e High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, Thermo) which greatly reduces ...
ORGANISM(S): Homo sapiens (Human) Macaca mulatta (Rhesus macaque) 
2021-07-30 | PXD023148 | Pride
Proteomics Mass spectrometry Plasma Depletion Sars-cov-2 Human Spin-column 
TranscriptomicsBAFF and CD4+ T cells are major survival factors for long-lived splenic plasma cells in a B-cell–depletion context
Previous data have suggested that B-cell–depletion therapy may induce the settlement of autoreactive long-lived plasma cells (LLPCs) in the spleen of patients with autoimmune cytopenia. To investigate this process, we used the AID-CreERT2-EYFP mouse model to follow PCs engaged in an immune response....
ORGANISM(S): Mus musculus 
2018-02-19 | E-MTAB-6449 | biostudies-arrayexpress
ProteomicsAS precipitation and protein A affinity chromatography for abundant plasma protein depletion
Plasma is a highly valuable resource for biomarker research since it is easy obtainable and contains a high amount of information on patient health status. Although advancements in the field of proteomics enabled analysis of the plasma proteome, identification of low abundant proteins remains challe...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-08 | PXD008969 | Pride
Plasma Mouse Abundant protein depletion 
MetabolomicsArginine depletion-induced autophagy and metabolic dysregulation are involved in the disease severity of hand, foot, and mouth disease
Amino acid metabolism provides significant insight into the development and prevention of many viral diseases. Therefore, the present study aimed to compare the amino acid profiles of hand, foot, and mouth disease (HFMD) patients with those of healthy individuals and to further reveal the molecular ...
2024-10-25 | MTBLS11341 | MetaboLights
ProteomicsPlasma samples processed with depletion and non depletion
Plasma samples processed with no depletion and most-abundant-proteins depletion procedures according to standard in-solution and single-pot, solid-phase-enhaced sample preparation (SP3) workflows using guanidyne hydrochloride and SDS lysis buffers for analysis with liquid chromatography mass spectro...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031919 | Pride
Plasma 
ProteomicsEvaluation of depletion of abundant plasma proteins for proteomics characterization of EVs
In this study, we aimed to investigate if the removal of abundant plasma proteins prior to EV isolation could improve plasma-derived EV characterization by LC-MS/MS by improving the proteome coverage. Plasma depletion was performed using single-use spin columns prior to SEC-based EV isolation. After...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD039240 | Pride
Ev Plasma depletion Dia Extracellular vesicles Lc-ms/ms 
ProteomicsBenchmarking plasma proteomics depletion and enrichment workflows prior to LC-MS/MS analysis
A plasma sample was prepared using multiple depletion and enrichment techniques in order to improve the depth of proteomics analyses.
ORGANISM(S): Homo sapiens (Human) 
2026-06-15 | PXD069187 | Pride
Astral Proteomics Depletion Plasma Enrichment 
ProteomicsAffinity capture enrichment versus Affinity depletion: A comparison of strategies for increasing coverage of low abundant human plasma proteins
present study, we evaluated four small molecule affinity-based probes based on agarose-immobilized benzamidine (ABA), O-Phospho-L-Tyrosine (pTYR), 8-Amino-hexyl-cAMP (cAMP), or 8-Amino-hexyl-ATP (ATP) for their ability to remove high-abundant proteins such as serum albumin from plasma samples thereb...
ORGANISM(S): Homo sapiens (Human) 
2020-08-27 | PXD020727 | Pride
Affinity enrichment Depletion Human plasma 
MetabolomicsA bovine plasma-derived serum substitute enables continuous manufacturing of cultivated fish biomass
Cultivated fish meat offers a sustainable solution to marine stock depletion, yet industrial scalability is limited by the prohibitive cost of fetal bovine serum (FBS). We developed a cost-effective bovine plasma-derived serum substitute (bPCPE-LTUS) through low-temperature ultrasonication. This phy...
2026-09-25 | MTBLS14288 | MetaboLights
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