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MetabolomicsPollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage
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ORGANISM(S): Arabidopsis thaliana 
ST002060 | MetabolomicsWorkbench
TranscriptomicsTranscription profiling of Arabidopsis cell-sorted, hydrated pollen grains vs those of flowers, leaves, seedlings and siliques
Upon germination, pollen forms a tube that elongates dramatically through female tissues in order to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We used Affymetrix Arabidopsis ATH1 Genome Ar...
ORGANISM(S): Arabidopsis thaliana 
2005-06-01 | E-MEXP-285 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis pollen germination and tube growth
Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step towards a detailed underst...
ORGANISM(S): Arabidopsis thaliana 
2008-10-25 | E-GEOD-6696 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis pollen and semi in vivo- and in vitro-grown pollen tubes.
Pollen tubes extend through pistil tissues and are guided to ovules where they release sperm for fertilization. Although pollen tubes can germinate and elongate in a synthetic medium, their trajectory is random and their growth rates are slower compared to growth in pistil tissues. Furthermore, inte...
ORGANISM(S): Arabidopsis thaliana 
2009-08-15 | E-GEOD-17343 | biostudies-arrayexpress
TranscriptomicsGene expression during pollen germination in Lily
cDNA microarray analysis of gene expression changes during pollen germination and tube growth in Lilium longiflorum L.
ORGANISM(S): Lilium longiflorum 
2010-06-11 | E-GEOD-2648 | biostudies-arrayexpress
TranscriptomicsGene expression in mature pollen and sperm cells versus young seedling as a vegetative sporophyte reference control
Sperm cells represent the male partner that fuses with the egg cell during fertilization in all multi-cellular eukaryotic organisms, and, in flowering plants, is a founder of both embryo and nutritive endosperm. We examined the transcriptome of Oryza sativa ssp. japonica using the Affymetrix 57K ri...
ORGANISM(S): Oryza sativa 
2012-04-29 | E-GEOD-17002 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis germinating pollen
In sexual reproduction, a proper communication and cooperation between male and female organs and tissue are essential for male and female gametes to unite. In flowering plants, female sporophytic tissues and gametophyte direct a male pollen tube towards an egg apparatus, which consists of an egg ce...
ORGANISM(S): Arabidopsis thaliana 
2009-08-09 | E-GEOD-12316 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of Arabidopsis thaliana pollen cDNA uncovers novel transcription and alternative splicing
Pollen grains of Arabidopsis thaliana contain two haploid sperm cells enclosed in a haploid vegetative cell. Upon germination, the vegetative cell extrudes a pollen tube that carries the sperm to an ovule for fertilization. Knowing the identity, relative abundance, and splicing patterns of pollen tr...
ORGANISM(S): Arabidopsis thaliana 
2013-05-25 | E-MTAB-4242 | biostudies-arrayexpress
TranscriptomicsSmall noncoding RNAs in tomato pollen
We used tomato pollen in order to identify pollen stage-specific small non-coding RNAs (sncRNAs) and their target mRNAs. We further deployed elevated temperatures to discern stress responsive sncRNAs. For this purpose high throughput sncRNA-sequencing was performed for three-replicated sncRNAs libra...
ORGANISM(S): Solanum lycopersicum 
2015-08-20 | E-MTAB-3830 | biostudies-arrayexpress
ProteomicsProteomic characterization of plasma membrane-enriched fraction from tobacco (Nicotiana tabacum L. cv Samsun) pollen tubes.
Pollen germination and subsequent pollen tube elongation are essential for successful land plant reproduction. These processes are achieved through well-documented activation of membrane trafficking and cell metabolism. Despite this, our knowledge of the dynamics of cellular phospholipids remains sc...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2022-10-27 | PXD037046 | Pride
Pollen tube Tobacco Tip growth Plasma membrane Polarity Pollen 
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