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TranscriptomicsIntegrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples pre-mRNA processing and mRNA stability [sequence data]
RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-...
ORGANISM(S): Homo sapiens 
2011-08-19 | E-GEOD-29779 | biostudies-arrayexpress
ProteomicsIdentification of mRNA processing complexes bound to histone H2A.2 3’ untranslated region
Processing of 3’ untranslated region (3’-UTR) of precursor messenger RNAs (pre-mRNA) is a fundamental step in mRNA maturation, where the mRNA transcript is cleaved at a specific site, located downstream the open reading frame of the encoded gene. The position of the cleavage site is determined by ci...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2022-12-09 | PXD034984 | Pride
Pre-mrna processing Processing factors Nanolc-ms/ms Mrna maturation 
ProteomicsMpe1 senses the polyadenylation signal in pre-mRNA to promote efficient cleavage and control polyadenylation
Crosslinking mass spectrometry dataset of the Mpe1 and Cft2 bound to the yeast CPF polymerase module. Sulfo-SDA is the crosslinker used.
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2023-01-12 | PXD027482 | JPOST Repository
ProteomicsIdentification of human mRNA processing complexes bound to histone H2A.2 3’ untranslated region
Processing of 3’ untranslated region (3’-UTR) of precursor messenger RNAs (pre-mRNA) is a fundamental step in mRNA maturation, where the mRNA transcript is cleaved at a specific site, located downstream the open reading frame of the encoded gene. The position of the cleavage site is determined by ci...
ORGANISM(S): Homo sapiens (Human) 
2022-12-09 | PXD027636 | Pride
3’-utr Mrnas Nanolc-ms/ms 3’ pre-mrna processing complexes 
ProteomicsProtein composition of catalytically active U7-dependent processing complexes assembled on histone pre-mRNA containing biotin and a photo-cleavable linker
To identify components of the U7 snRNP, we used cleavage-resistant histone pre-mRNA containing biotin and a photo-sensitive linker and purify processing complexes from Drosophila and mouse nuclear extracts.
ORGANISM(S): Mus musculus (Mouse) Drosophila melanogaster (Fruit fly) 
2018-03-08 | PXD008917 | Pride
Flash  lsm11 Histone pre-mrna Photo-cleavable linker 3’ end processing U7 snrnp  slbp 
TranscriptomicsProtein arginine methyltransferase AtPRMT5 is involved in pre-mRNA splicing in Arabidopsis thaliana
In this study, we analyzed the Arabidopsis homologue of PRMT5, AtPRMT5M-bM-^@M-^Ys function in RNA processing. RNA-seq analyses revealed that AtPRMT5 is involved in a subset of pre-mRNA splicing. Several RNA processing factors involved in regulating flowering time were validated that the correspondi...
ORGANISM(S): Arabidopsis thaliana 
2010-10-07 | E-GEOD-21323 | biostudies-arrayexpress
ProteomicsReconstitution of 3’-processing of mammalian pre-mRNA reveals a central role of RBBP6
The 3’ ends of almost all eukaryotic mRNAs are generated in an essential two-step processing reaction, endonucleolytic cleavage of an extended precursor followed by the addition of a poly(A) tail. By reconstituting the reaction from overproduced and purified proteins, we provide a minimal list of fo...
ORGANISM(S): Homo sapiens (Human) 
2022-03-04 | PXD031227 | Pride
Cpsf 3’-processing Xl-ms Rbbp6 
TranscriptomicsUBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells
UBL5 is an atypical ubiquitin-like protein, whose function in metazoans remains largely unexplored. We show that UBL5 is required for sister chromatid cohesion maintenance in human cells. UBL5 primarily associates with spliceosomal proteins, and UBL5 depletion decreases pre-mRNA splicing efficiency,...
ORGANISM(S): Homo sapiens 
2014-08-05 | E-GEOD-59376 | biostudies-arrayexpress
GenomicsRBBP6 anchors pre-mRNA 3’ end processing to nuclear speckles for efficient gene expression
RBBP6 anchors pre-mRNA 3’ end processing to nuclear speckles for efficient gene expression
PRJNA1082279 | ENA
GenomicsWDR82 Code Coordinates Transcription and pre-mRNA 3’-End Processing Through Regulated Phosphorylation
WDR82 Code Coordinates Transcription and pre-mRNA 3’-End Processing Through Regulated Phosphorylation
PRJNA1331813 | ENA
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