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ProteomicsEvaluation of the technical reproducibility of serum proteome sample preparation methods to support clinical proteomics applications
Evaluation of the efficiency and reproducibility of sample prepration methods using iST-BCT kit from PreOmics or SMART Trypsin Kit (Thermo Fisher) with in-house Rapigest protocol in human serum samples prior to mass spectrometry analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-04-07 | PXD051002 | Pride
Serum  proteomics  biomarker  mass spectrometry  rapigest sf  ist-bct  smart digest trypsin 
ProteomicsEvaluating Protein Enrichment Methods to Improve Biomarker Discovery in Equine Neurological Disorders
This study evaluated and compared three sample preparation strategies for proteomic analysis of equine cerebrospinal fluid (CSF): native in-solution digestion, the ProteoMiner™ Small-Capacity Enrichment Kit (Bio-Rad®), and the PreOmics Enrich-iST™ Kit (PreOmics®). CSF is in direct contact with the c...
ORGANISM(S): Equus caballus (Horse) 
2026-04-13 | PXD063239 | Pride
Cerebrospinal fluid (csf)  proteomics  native digestion  proteominer™ small-capacity enrichment kit  preomics enrich-ist™  liquid chromatography with tandem mass spectrometry (lc-ms/ms) 
ProteomicsComparative evaluation of different sample preparation workflows for reproducible, quantitative, and in-depth analysis of urine proteomics
The growing field of urinary proteomics has provided a promising opportunity to identify biomarkers useful for diagnosis and prognostication of a number of diseases. Urine is abundant and readily collected in a non-invasive manner which makes it eminently available for testing, however sample prepar...
ORGANISM(S): Homo sapiens (Human) 
2020-03-06 | PXD016273 | Pride
Reproducibility Urine proteomics Mass spectrometry Preomics ist Mstren Sample preparation Label-free S-trap 
ProteomicsImpact of Sample Preparation Strategies on The Quantitative Accuracy of Low-Abundance Serum Proteins in Shotgun Proteomics
Serum proteomics plays a crucial role in biomarker discovery and disease research, yet the selection of an optimal sample preparation method remains challenging. Evaluating the accuracy of protein quantitation is of major importance and a vital part of a benchmarking study in proteomics, since clini...
ORGANISM(S): Homo sapiens (Human) 
2025-09-15 | PXD062785 | Pride
Reproducibility Quantitative accuracy Serum proteomics Depth of coverage 
ProteomicsHow blood matrices and sample preparation can influence blood marker discovery
While plasma and serum are increasingly being studied by using high throughput proteomics, it remains largely unexplored how different types of blood materials collected in the routine clinical diagnostics laboratories influence protein marker discovery. We compared the LC-MS/MS proteomic profiles o...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD066304 | Pride
Plasma Speed Mass spectrometry Enrich-ist Serum Lc−ms Ist R package mpwr Data-independent acquisition Perca Mag-net 
ProteomicsComparative analysis of MS sample preparation methods
We compare the most commonly used proteomic sample preparation strategies such as classical in solution digest protocols, SPEED, FASP, STrap, SP3, as well as the commercially available kits iST (PreOmics) and EasyPep (Thermo Scientific) in their efficacy to extract proteomes from HeLa cells. We find...
ORGANISM(S): Homo sapiens (Human) 
2022-09-06 | PXD030406 | Pride
Hela Human Sample preparation Lc-ms/ms 
ProteomicsWnt5a and Vangl1/2 signaling regulates the position and direction of lung branching through the cytoskeleton and focal adhesions
Whole cell proteomics on WT and VdKO cell lines prepared with preomics iST kit. 200 ng injections with 90 min gradient.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-07-08 | MSV000089851 | MassIVE
UnknownImpact of Sample Preparation Strategies on the Quantitative Accuracy of Low-Abundance Serum Proteins in Shotgun Proteomics.
Not available
S-EPMC12418494 | biostudies-literature
ProteomicsTargeting Ras-, Rho-, and Rab-Family GTPases via a Conserved Cryptic Pocket
The family of Ras-like GTPases consists of over 150 different members, regulated by an even larger number of guanine exchange factors (GEFs) and GTPase-activating proteins (GAPs) which comprise cellular switch networks that govern cell motility, growth, polarity, protein trafficking, and gene expres...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054414 | Pride
Gtpase 
ProteomicsGeorge...Flynn et al 2023 Mass Spectrometry Data
Proximity labeling studies for isotype control or NPM1. Proteins were biotinylated, enriched, reduced, and alkylated. Peptides were prepared and desalted using Preomics iST. Samples were loaded onto a Bruker NanoElute and separated/analyzed as described in the manuscript.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-06-19 | MSV000092211 | MassIVE
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