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TranscriptomicsT cell help controls the speed of the cell cycle in germinal center B cells.
The germinal center (GC) is a microanatomical compartment wherein high-affinity antibody-producing B cells are selectively expanded. B cells proliferate and mutate their antibody genes in the dark zone (DZ) of the GC and are then selected by T cells in the light zone (LZ) on the basis of affinity. H...
ORGANISM(S): Mus musculus 
2015-08-12 | E-GEOD-71295 | biostudies-arrayexpress
ProteomicsSample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid Extraction
We report a universal detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Bacillus cereus ATCC 10987 Escherichia coli Staphylococcus aureus 
2019-11-25 | PXD011189 | Pride
Speed Sample preparation Quantitative proteomics 
TranscriptomicsTranscriptome analysis of cells of different cycling speed during Yamanaka reprogramming
Reprogramming somatic cells to induced pluripotency by Yamanaka factors is usually slow and inefficient, and is thought to be a stochastic process. We identified a privileged somatic cell state, from which acquisition of pluripotency could occur in a non-stochastic manner. Subsets of murine hematopo...
ORGANISM(S): Mus musculus 
2014-02-12 | E-GEOD-53074 | biostudies-arrayexpress
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030797 | Pride
Speed Plasma Kidney Sheep Tripletof S-trap Dda 
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030796 | Pride
Speed Plasma Kidney Sheep Swath-ms Tripletof S-trap 
GenomicsControl of DNA replication fork speed
An Additional Replication Origin Causes Cell Cycle Specific DNA Replication Fork Speed.
PRJEB86591 | ENA
GenomicsCellular NAD+ availability constrains developmental speed in the Drosophila eye
Cellular NAD+ availability constrains developmental speed in the Drosophila eye
PRJNA1251508 | ENA
GenomicsHigh-speed fluorescence image-enabled cell sorting
High-speed fluorescence image-enabled cell sorting
PRJNA705647 | ENA
GenomicsOrganismal Benefits of Transcription Speed Control at Gene Boundaries
Organismal Benefits of Transcription Speed Control at Gene Boundaries
PRJNA550085 | ENA
GenomicsDNA replication fork speed Acts as A Pacer in Cortical Neurogenesis
DNA replication fork speed Acts as A Pacer in Cortical Neurogenesis
PRJNA1175628 | ENA
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