Cornea transplantation is a widely performed procedure globally. However, storing donor corneas for extended periods in Optisol-GS storage medium at 4 ℃ can lead to reduced endothelial cell viability, causing severe stromal edema and opacification. This negatively impacts transplantation success. Th...
This dataset contains single-cell RNA sequencing (scRNA-seq) data generated from a multicellular human liver-on-chip model to characterise cell-type-specific transcriptional responses to hypothermic preservation. The organ-on-chip system comprised four cell populations: patient-derived hepatic organ...
When studying gene expression in microbe-animals symbioses collected in the field it is essential to quickly and efficiently preserve in situ symbiont and host protein abundance patterns. One of the most commonly used sample preservation methods for samples targeted for proteomic analyses is flash f...
Human cochlear organoids were derived from human pluripotent stem cell–based differentiation and maintained in CHIR99021-containing proliferative medium (EFI). Organoids were cultured under proliferative conditions for 10 days, after which they were transduced with 3 types of adeno-associated virus ...
The present study explores the advantages of enriching the freezing medium with membrane lipids and antioxidants in improving the outcome of prepubertal testicular tissue cryopreservation. For the study, testicular tissue from Swiss albino mice of prepubertal age group (2 weeks) was cryopreserved by...
