Sort   by:  
 Page size 
ProteomicsProtein aggregation capture on microparticles enables multi-purpose proteomics sample preparation
Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-03-05 | PXD011677 | Pride
Microparticles Mass spectrometry Secretomics Proteomics Sample preparation Phosphoproteomics Magnetic beads 
UnknownProtein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation.
Not available
S-EPMC6495262 | biostudies-literature
GenomicsNGLY1 mutations cause protein aggregation in human neurons
NGLY1 mutations cause protein aggregation in human neurons
PRJNA810420 | ENA
ProteomicsAbundance analysis of chromatin-residing proteins during lineage conversion using protein aggregation capture (PAC)
We performed abundance analysis of chromatin-residing proteins in BLaER1 cells during the early stages of B-cell-to-macrophage transdifferentiation. We employed protein aggregation capture (PAC) to analyze chromatin-residing protein abundances at 0, 6 and 24 h after induction of transdifferentiation...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD076495 | Pride
Mass spectrometry Transdifferentiation Human Chromatin Blaer1 
TranscriptomicsThe ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
Recent studies highlight the importance of translational control in determining protein abundance, underscoring the value of measuring gene expression at the level of translation. We present a protocol for genome-wide, quantitative analysis of in vivo translation by deep sequencing. This ribosome pr...
ORGANISM(S): Homo sapiens 
2012-07-26 | E-GEOD-37744 | biostudies-arrayexpress
UnknownSuppressing mutation-induced protein aggregation in mammalian cells by mutating residues significantly displaced upon the original mutation.
Not available
S-EPMC4504021 | biostudies-literature
ProteomicsDifferences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques.
The goal of proteomics experiments is to identify proteins to observe changes in cellular processes and diseases. One challenge in proteomics is the removal of contaminants following protein extraction, which can limit protein identifications. Single-pot, solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-20 | MSV000094130 | MassIVE
GenomicsPromoter Capture-HiC in Ring1a KO and Ring1a/b dKO mouse ES cells
Genome organization influences transcriptional regulation by facilitating interactions between gene promoters and distal regulatory elements. To analyse distal promoter contacts mediated by the PRC1 complex we used Capture Hi-C (CHi-C) to enrich for promoter-interactions in a HiC library in Ring1a K...
ORGANISM(S): Mus musculus 
2015-08-01 | E-MTAB-3109 | biostudies-arrayexpress
ProteomicsCo-opting templated aggregation to selectively degrade pathogenic tau assemblies
Protein aggregation causes a wide range of neurodegenerative diseases. Targeting and removing aggregates, but not the functional protein, is a considerable therapeutic challenge. Here we describe a therapeutic strategy called ‘RING-Bait’ which employs an aggregating protein sequence combined with an...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD052897 | Pride
Proteinopathies 
ProteomicsCo-translational protein aggregation and ribosome stalling as a novel broad-spectrum antibacterial mechanism
Drug- resistant bacteria pose are an urgent global threat health threatto global health, necessitatingrequiring the development of antibacterial compounds with a novel mode of action. Protein biosynthesis accounts for up to half of the energy expenditure of bacterial cells and consequently inhibitin...
ORGANISM(S): Escherichia coli 
2025-02-11 | PXD056779 | Pride
Antimicrobial peptide Protein aggregation 
Sort   by:  
 Page size