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TranscriptomicsTcRBP40 pull-down ribonomics
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
2012-06-30 | E-MEXP-3057 | biostudies-arrayexpress
ProteomicsFilaminC pull-down experiment from myotube lysates
This project was designed to identify a difference in the binding affinity of FILIP1 to different FLNc variants. To this end FLNc domain 1-3 and FLNc d18-21 were used as bait for a pull-down analysis.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-25 | PXD017670 | Pride
Pull-down Myotube lysate Flnc 
ProteomicsRNA pull down experiment for vA-202 (ANAPC1P2) lncRNA
This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
Human Rna pull down Orbutrap ms 
ProteomicsHaCaT ACSL4mRNA probe pull-down MS
ACSL4mRNA probe was designed for pull-down experiments in HaCaT lysates
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD052755 | Pride
Hacat acsl4mrna pull-down ms 
Proteomicspull-down of PpSerpin-1 isoforms
proteomic identification of pull-down gel slices of PpSerpin-1 isoforms
ORGANISM(S): Pieris rapae 
2023-08-05 | PXD044199 | Pride
Pteromalus puparum Serpin Pull-down Alternative splicing 
GenomicsRNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
PRJNA561512 | ENA
GenomicsDeep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaena sp. strain PCC 7120
We employed chromatin pull-down and deep sequencing to globally identify HetR DNA targets in vivo at 6 hours after fixed-nitrogen deprivation. We identified novel DNA binding targets of tagged HetR-6xHis and defined a consensus HetR binding site from these HetR target sequences. Chromatin pull down ...
ORGANISM(S): Nostoc sp. PCC 7120 
2013-10-31 | E-GEOD-51886 | biostudies-arrayexpress
GenomicsProteomic and Genomic Analysis of transcription factor TFII-I reveals insight into the response to cellular stress
We expressed biotin-tagged transcription factor TFII-I (GTF2I) in K562 cells and subjected crosslinked chromatin to streptavidin mediated pull down. We subjected the isolated DNA to Illumina sequencing. We identified 18920 TFII-I binding peaks. Nearest gene analysis revealed 5886 genes associated w...
ORGANISM(S): Homo sapiens 
2014-05-14 | E-GEOD-51065 | biostudies-arrayexpress
ProteomicsProtein idetification by using DNA pull-down & MS assay
Identification of proteins binding to the promoter of hrpR gene in Pseudomonas syringae using DNA pull-down & MS assay
ORGANISM(S): Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 
2021-03-23 | PXD023722 | Pride
Promoter Dna pull-down Lc-ms/ms 
ProteomicsGST Pull-down-MS identify candidate host factors that interact with CHIKV nsP4
The Chikungunya virus (CHIKV) is a single positive-stranded RNA virus transmitted by mosquitoes. More than 40 countries have reported cases of CHIKV infection to varying degrees. Currently, there is no licensed specific antiviral drug or vaccine for CHIKV in the world. In this study, we have identif...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD047753 | Pride
Chikungunya virus  nonstructural protein 4 
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