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ProteomicsAcquisition of embryogenic competence during protoplast development of Fagopyrum
Reprogramming in protoplast cultures is a key step for acquiring embryogenic competence by dedifferentiated cells, thus leading to plant regeneration. Therefore, to better understand factors trigging the processes, this work focuses on analysing proteomes, cell wall composition and changes in the ex...
ORGANISM(S): Fagopyrum Esculentum (ncbitaxon:3617) Fagopyrum Tataricum (ncbitaxon:62330) 
MSV000095861 | MassIVE
TranscriptomicsSingle-protoplast and single-nucleus sequencing of the leaves of two C4 species: Gynandropsis gynandra and Flaveria bidentis
In this study, we optimized protocols for single-nucleus and single-protoplast RNA sequencing (RNA-seq) to generate high-resolution transcriptome atlases for two C4 plant species, Gynandropsis gynandra and Flaveria bidentis. Using Chromium (10X Genomics) technology, we generated single-nucleus and ...
ORGANISM(S): Flaveria bidentis 
2025-08-15 | E-MTAB-15121 | biostudies-arrayexpress
ProteomicsBacterial Protoplast-Derived Nanovesicles Carrying CRISPR Cas9 Tools Efficiently Re-educate Tumor-Associated Macrophages for Cancer Immunotherapy
we developed an in vivo CRISPR-Cas9 genome editing system that targets tumor-associated macrophages (TAMs) via bacterial protoplast-derived nanovesicles (NVs) decorated with a pH-responsive PEG conjugated polymer and galactosamine-conjugated ligand
ORGANISM(S): Escherichia Coli Bl21(de3) 
PXD045507 |
TranscriptomicsTranscriptome of Protoplasts Reprogrammed into Stem Cells in Physcomitrella patens
We obtained genome-wide digital gene expression tag profiles within the first three days of P. patens protoplast reprogramming. At four time-points during protoplast reprogramming, the transcript levels of 4827 genes changed more than four-fold and their expression correlated with the reprogramming ...
ORGANISM(S): Physcomitrella patens 
2015-02-28 | E-GEOD-36117 | biostudies-arrayexpress
GenomicsProtoplast data: suberized exodermis is required for tomato drought tolerance
Protoplast data: suberized exodermis is required for tomato drought tolerance
PRJNA875392 | ENA
TranscriptomicsProtoplast data: suberized exodermis is required for tomato drought tolerance
This experiment aimed to identify protoplast-induced genes in tomato seedling, for future use in single cell datasets.
ORGANISM(S): Solanum lycopersicum 
2023-10-01 | GSE212404 | GEO
Transcriptomicstranscriptome profiling from a protoplast culture of arabidopsis thaliana-From protoplast to plant : transcrip...
ra03-03_protoplats - transcriptome profiling from a protoplast culture of arabidopsis thaliana - transcriptome profiling and comparison of 6 status of differentiation - protoplasts were extracted from plantlet cultivated during 2 weeks then placed in a culture midium which stimulate cell division. P...
ORGANISM(S): Arabidopsis thaliana 
2007-06-20 | GSE7984 | GEO
GenomicsSingle-nucleus RNA sequencing (snRNA-seq) of protoplast-isolated nuclei from Populus developing xylem (SDX)
Single-nucleus RNA sequencing (snRNA-seq) of protoplast-isolated nuclei from Populus developing xylem (SDX)
PRJEB107759 | ENA
UnknownEmbryogenic Stem Cell Identity after Protoplast Isolation from Daucus carota and Recovery of Regeneration Ability through Protoplast Culture.
Not available
S-EPMC9570137 | biostudies-literature
UnknownOptimization of protoplast regeneration in the model plant Arabidopsis thaliana.
Not available
S-EPMC7901198 | biostudies-literature
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